Amphisphaeriales » Pseudotruncatellaceae » Pseudotruncatella

Pseudotruncatella camporesii

Pseudotruncatella camporesii Goonas. & Hyde, in Hyde et al., Fungal Diversity 100: 221 (2020)

Index Fungorum number: IF556928; Facesofungi number: FoF 06822

Etymology: Named after the collector Erio Camporesi.

Holotype: MFLU 150796

Saprobic on dead stem of Helichrysum sp. Sexual morph: Undetermined. Asexual morph: Conidiomata black, shiny, raised or flattened, rounded, 170–250 μm high, 280–400 μm wide (= 200 × 340 μm, n = 10) acervular to pycnidioid, scattered or gregarious, uni-loculate, globose or oval with a flattened base, semi-immersed to superficial, sometimes erumpent. Pycnidial walls comprising several indistinct cell layers of golden brown to pale brown cells of textura angularis to textura prismatica, and towards the upper part of the fruiting body, more of textura prismatica and textura globulosa. Conidiophores arising from the basal and lateral cells of the conidioma cavity, cylindrical, reduced to conidiogenous cells, unbranched, smooth, hyaline. Conidiogenous cells 10–38 × 1.5–3 μm (= 26 × 2 μm, n = 20) cylindrical, hyaline, smooth, determinate, holoblastic, proliferating percurrently at apex, aseptate. Conidia 28–35 × 6–8.5 μm, (x̄ = 31 × 7.3 μm, n = 40), fusoid, straight to slightly curved, 2-septate, slightly constricted at second septum from base, basal cell 5.8–12 × 2.5–3.5 μm (= 9 × 2.8 μm), 15–30 × 1.5–2 μm, narrowly obconic, truncate at base, hyaline, smooth-walled, thin-walled, second and third cells from the base, together 18.5–26 × 5.5–9 μm (= 22 × 6.8 μm), dark brown, smooth-walled, thickwalled, slightly guttulate, fusoid, bearing three tubular apical appendages, 18.6–25.8 μm (= 23.2 μm) long, smooth, tubular, unbranched.

Culture characteristics: Colonies on PDA growing up to 20 mm in diam. at 16–18 °C after seven days. Colony irregular in shape, flat to effuse, surface smooth, with undulate margin, yellowish-white, reverse grey.

Material examined: ITALY, Province of Forlì-Cesena [FC], near Passo delle Forche-Galeata, on dead stem of Helichrysum sp. (Asteraceae), E. Camporesi, 16 July 2014, IT 2001 (MFLU 15–0796, holotype); MFLU 15–0797 = KUN-HKAS 96293, isotype, ex-type living culture, MFLUCC 15–0562.

GenBank Numbers: MN648203.

Note: Pseudotruncatella arezzoensis and P. camporesii are reported on Helichrysum sp. as saprobes. The two species can be distinguished by their conidiomata dimensions (230–275 μm high, 255–280 μm wide versus 170–250 μm high, 280–400 μm wide). Pseudotruncatella arezzoensis has pycnidioid, immersed to semi-immersed conidiomata, while, in P. camporesii they are acervular to pycnidioid, semi-immersed to superficial (Perera et al. 2018). Pseudotruncatella camporesii has longer conidiogenous cells and larger conidia (28–35 × 6–8.5 μm versus 20–25 × 5.4–6.5 μm, Perera et al. 2018). Furthermore, P. arezzoensis produces 3-septate conidia with no constriction at the septum while the conidia of P. camporesii are 2-septate and slightly constricted at the septum. Likewise, P. bolusanthi has 2-septate conidia showing constriction at mid-septum (Crous et al. 2019). However, the latter has smaller conidiomata of 150–200 μm diam., with 0–3-septate, branched conidiophores and shorter conidia (17–20 × 6.5–7 μm), with one apical appendage (Crous et al. 2019). In the phylogenetic analysis our new isolate clustered with P. arezzoensis with high bootstrap support (96% ML, 1.00 BYPP).

 

Figure x. Pseudotruncatella camporesii (MFLU 150796, holotype). a Conidiomata on dead stem of Helichrysum sp. b A close-up of conidioma. c Cross section of conidioma. d Pycnidial wall. e Developing conidia from conidiogenous cells. f–h Conidia. i Germinating spore. Scale bars c = 50 μm, de, gh = 10 μm, fi = 20 μm.

 

References:

 

Crous PW, Carnegie AJ, Wingfield MJ, Sharma R et al. 2019 – Fungal Planet description sheets: 868–950. Persoonia 42, 291–473.

Hyde KD, Dong Y, Phookamsak R, Jeewon R et al. 2020 – Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 16, 1–273.

Perera RH, Maharachchikumbura SS, Hyde KD, Bhat DJ et al. 2018 – An appendage-bearing coelomycete Pseudotruncatella arezzoensis gen. and sp. nov. (Amphisphaeriales genera incertae sedis) from Italy, with notes on Monochaetinula. Phytotaxa 338, 177–188.

 

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