Broomella rosae

Broomella rosae Wanas., Camporesi, E.B.G. Jones & K.D. Hyde, in Wanasinghe et al., Fungal Diversity: 10.1007/s13225-018-0395-7, [191] (2018)

Index Fungorum number: IF 554222; Facesofungi number: FoF 04065

Etymology: The specific epithet reflects the host genus Rosa.

Holotype: MFLU 16-0244.

Saprobic on spines of Rosa canina. Sexual morph: Ascomata 150–250 μm high 200–300 μm diam. (x̄ = 195.5 × 265.9 μm, n = 10), solitarily, often with a brown, immersed or semi-immersed in the host tissues, black, with smooth to somewhat rough surface, globose, with flattened base. Ostiole central, papillate, with circular outline, ostiolar neck comprising brown setae, ostiolar cannel filled with hyaline periphyses. Peridium 5–10 μm thick at the base, 20–30 μm near the ostiole, outer layers thin-walled, comprising dark brown cells of textura angularis to textura intricata, inner layers composed of hyaline to brown, thin-walled, flat cells of textura angularis. Hamathecium comprising numerous, 2.5–3.5 μm (n = 20) wide, filamentous, septate, paraphyses. Asci 70–80 × 7–9 μm (x̄ = 75.6 × 8.1 μm, n = 30), 8-spored, unitunicate, cylindrical to cylindric-clavate, pedicellate, apical ring inconspicuous, J+. Ascospores 12–15 × 6–8 μm (x̄ = 13.6 × 6.9 μm, n = 40), overlapping uniseriate, ellipsoid, mostly symmetrical, sometimes one side flattened, 1-septate, not constricted at the septum, initially hyaline, becoming yellowish-brown to brown at maturity, with conically rounded ends. Asexual morph: Undetermined.

Material examined: ITALY, Forli-Cesena [FC] province, Tessello, Cesena, on dead aerial spines of Rosa canina L. (Rosaceae), 5 March 2015 Erio Camporesi IT 2401 (MFLU 16-0244, holotype).

GenBank: ITS: MG828874, LSU: MG828990

Notes: During our investigation on the diversity of microfungi in Italy, an isolate (MFLU 16-0244) was recovered from Rosa spines in Forli-Cesena Province. This new isolate shares a close phylogenetic affinity to Broomella vitalbae, Hyalotiella spartii, and Truncatella angustata. Based on priority, the generic name Broomella is applied herein for the new isolate. However, morphologically it differs from the genera Broomella, Hyalotiella, and Truncatella, with 1-septate ellipsoidal ascospores, lack of appendages, and asci with a J+, apical ring. We did not obtain an isolate and therefore we isolated DNA directly from the fruiting bodies.