Amphisphaeriales » Sporocadaceae » Pestalotiopsis

Pestalotiopsis italiana

Pestalotiopsis italiana Maharachch., Camporesi & K.D. Hyde, in Liu et al., Fungal Diversity: 10.1007/s13225-015-0324-y, [16] (2015)

Index Fungorum number: IF 550944; Facesofungi number: FoF 00458

Etymology: named after the country, where it was collected.

Holotype: MFLU 14–0214.

Saprobic on Cupressus glabra. Sexual morph: Undetermined. Asexual morph: Conidiomata pycnidial

in culture on PDA, globose, scattered or gregarious and confluent, semi-immersed, dark brown, up to

200 μm diam. Conidiophores septate near the base, branched, sub-cylindrical. Conidiogenous cells discrete, sub-cylindrical or ampulliform to lageniform, smooth-walled, percurrently proliferating 1–3 times. Conidia 26–35 × 8–11 μm (x̄ = 30 × 9.6 μm, n = 20), ellipsoid, straight to slightly curved, 4-septate, basal cell conic with obtuse end, hyaline, thin-walled and verruculose, 5–7 μm long (x̄ = 6 μm), with three median cells, doliform to cylindrical, with thick verruculose walls, constricted at the septa, concolourous, olivaceous, septa and periclinal walls darker than the rest of the cell, wall rugose, together 18–28 μm long (x̄ = 23 μm) second cell from base 5.5–8.5 μm (x̄ = 6.7 μm); third cell 6–9 μm (x̄ = 7 μm); fourth cell 6–9μm (x̄ = 7.7 μm); apical cell hyaline, conic to sub-cylindrical 4–6.5 μm long (x̄ = 4.9 μm); with 2–5 tubular apical appendages (mostly 3–4), arising from the apex of the apical cell (rarely 1 appendage arising from just above the septum separating upper median and apical cell), 20–40μm long (x̄ = 32 μm); basal appendage present 6–10 μm (x̄ = 7μm).

Culture characteristics: Colonies on PDA reaching 50–60 mm diam. after 7 d at 25 °C, with an undulate edge, whitish to pale grey-coloured, with dense aerial mycelium on surface, and black, gregarious conidiomata; reverse similar in colour.

Material examined: ITALY, Province of Forlì-Cesena, Camposonaldo, Santa Sofia, on dead twigs of Cupressus glabra, 24 November 2011, E. Camporesi IT051 (MFLU 14–0214, holotype); ex-type living culture MFLUCC 12–0657.

GenBank Numbers: ITS: KP781878, TEF: KP781881, TUB: KP781882.

Notes: Pestalotiopsis italiana was collected on dead twigs of Cupressus glabra in Italy, and forms a sister clade to P. brassicae (CBS 170.26), P. hollandica (CBS 265.33) and P. verruculosa (MFLUCC 12–0274) which were isolated from seeds of Brassica napus in New Zealand, Sciadopitys verticillata in the Netherlands and Rhododendron sp. in China, respectively. Pestalotiopsis italiana overlaps morphologically with P. verruculosa. However, in the phylogenetic analyses it formed a distinct lineage apart from P. verruculosa and geographically they are clearly distinct. 

 

Figure x. Pestalotiopsis italiana (holotype) a Conidiomata on PDA. b, c Conidiogenous cells. d–g Conidia. Scale bars: b–g=10μm.

 

References:

 

Liu JK, Hyde KD, Jones EG, Ariyawansa HA et al. 2015 – Fungal diversity notes 1–110: taxonomic and phylogenetic contributions to fungal species. Fungal diversity 72, 1–97.

 

Last update: 10 Feb 2021

 

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