Amphisphaeriales » Sporocadaceae » Sporocadus

Sporocadus rosigena

Sporocadus rosigena F. Liu, L. Cai & Crous, in Liu, Bonthond, Groenewald, Cai & Crous, Stud. Mycol. 92: 402 (2018) [2019]

Replaced synonym: Seimatosporium rosicola Wanas., Goonas., Camporesi, & K.D. Hyde, in Wanasinghe et al., Fungal Diversity: 10.1007/s13225-018-0395-7, [193] (2018)

Index Fungorum number: IF 828418; Facesofungi number: FoF 04066

Description taken from Wanasinghe et al. (2018).

Saprobic on spines of Rosa spp. Sexual morph: Ascomata 120–160 μm high 220–280 μm diam. (x̄ = 140.2 × 248.7 μm, n = 10), solitarily, immersed or semi-immersed, black, globose, ostiolate. Ostiole central, papillate, with circular outline, filled with pale-brown to brown cells. Peridium 5–10 μm thick at the base, 15–25 μm near the ostiole, outer layers heavily pigmented, fusing outwardly with the host cells, thin-walled, comprising dark brown cells of textura angularis to textura intricata, inner layers composed of hyaline to brown, thin-walled, flat cells of textura angularis. Hamathecium comprising numerous, 3.5–4 μm (n = 20) wide, filamentous, septate, paraphyses. Asci 50–70 × 8–12 μm (x̄ = 62.6 × 10.2 μm, n = 30), 8-spored, unitunicate, cylindrical to cylindric-clavate, pedicellate, apical ring inconspicuous, J+. Ascospores 11–13 × 5–7 μm (x̄ = 12.1 × 6.5 μm, n = 40), overlapping uniseriate, ellipsoid, pale brown, mostly symmetrical, muriform, 3-transversely septate, with 1-vertical septum, not constricted at the septa, initially hyaline, becoming yellowishbrown to brown at maturity, with rounded ends. Asexual morph: Conidiomata 200–350 μm diam., 80–120 μm high, pycnidial, solitary, semi-immersed to superficial, unilocular, conic or subglobose with flattened base, dark brown to black. Conidiomata wall (4.5–8 μm) not well defined, comprising brown, thin-walled cells of textura angularis, with lighter cells at the base fusing into the host tissue. Conidiophores arising from the cells at the base, cylindrical, unbranched, hyaline, smooth, up to 30 μm long, surrounded by many filiform, hyaline filaments. Conidiogenous cells enteroblastic, phialidic, integrated, hyaline, determinate, smooth, formed from the innermost layer of the conidiomata wall. Conidia (15.5–)12–14 × 5– 7.5(–9) μm (x̄ = 13 × 6.5 μm, n = 35), variable, mostly obovoid or broad fusiform, straight, initially hyaline, pale to moderate brown at maturity, with 3 transverse, thick, darker septa, occasionally constricted at the septa, often obtuse at both ends, or well rounded, smooth-walled, basal cell conical with a truncate base, pale brown or hyaline, 1.2–2.6 μm long (x̄ = 2 μm), with two median cells doliiform, pale brown to brown, together 5.6–7.7 μm long (x̄ = 6.5 μm), second cell from the base 1.2–3 μm long (x̄ = 2.5 μm), third cell from the base 1.5–3.5 μm long (x̄ = 2.6 μm), apical cell conical with obtuse or rounded apex, pale brown to brown, 1.7–3.8 μm long (x̄ = 2.8 μm).

Culture characteristics: Colonies on PDA reaching 1.5–2 cm diam. after 7 days at 16–18 °C, filamentous, circular, flat with entire margin, from above white, pale yellow at the center; reverse white.

Material examined: ITALY, Province of Forli-Cesena [FC], near Corverselle, Castrocaro Terme e Terra del Sole, on dead aerial spines of Rosa canina L. (Rosaceae), 30 November 2014, Erio Camporesi IT 2263 (MFLU 16-0239, holotype), ITALY, Forlı `-Cesena Province, near San Benedetto in Alpe, on spines of Rosa canina L. (Rosaceae), 21 October 2014, Erio Camporesi IT 2187 (MFLU 15-0806), ibid. (MFLU 15-0807–12), living culture MFLUCC 15-0564 = KUMCC 17-0020.

GenBank: ITS: MG828958, MG828959, LSU: MG829069, MG829070

Notes: Wanasinghe et al. (2018) introduced the taxon as Seimatosporium rosicola (Holotype: MFLU 16-0239) based on morphology and multi-gene phylogenetic analyses. However, the study of Liu et al. (2019) provided a nomenclatural novelty as Sporocadus rosigena by replaced the synonym. see original note Wanasinghe et al. (2018).