Botryosphaeria dothidea

Botryosphaeria dothidea (Moug.) Ces. & De Not., Comm. Soc. crittog. Ital. 1(fasc. 4): 212 (1863)

Index Fungorum number: IF183247; Facesofungi number: FoF 03512

Saprotrophic on dead and aerial branches of Vitis vinifera. Sexual morph: Ascomata 200−350 μm diam., 175−280 μm high, erumpent, aggregated or solitary, globose with a central ostiole. Peridium comprising 5–10 layers of textura angularis, outer region of dark brown cells, inner region of 2–4 layers of hyaline cells lining the locule. Hamathecium comprising 2–4 μm wide cells. Pseudoparaphyses filiform, septate, constricted at the septa, rarely branched. Asci 60–120 × 15–21 μm (x̄ = 95 × 18 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical, forming between pseudoparaphyses, pedicellate, rounded at the apex, with an ocular chamber. Ascospores 20–24×6–8 μm (x̄ =23 × 6 μm, n=30), fusoid to ovoid, sometimes with tapered ends and appearing spindle-shaped, biseriate in the ascus. Asexual morph: Conidiomata stromatic, 250–450 μm (x̄ =320 μm, n=20). Paraphyses hyaline, septate, up to 100 μm long, tapering to acutely rounded apices. Conidiophores hyaline, cylindrical, smooth. Conidiogenous cells hyaline, sub-cylindrical, 4–16 × 2–5 μm (x̄ =8 μm, n=20). Conidia 15–26 × 5–9 μm (x̄ =18 μm n=40), hyaline, unicellular, narrowly fusiform, with a sub-truncate to bluntly rounded base, forming a septum before germination, smooth-walled with a granular content.

Culture characteristics: Colonies olivaceous, becoming grey, reverse black. Mycelial mat moderately dense, margin smooth. Colony on PDA reaching 50 mm diam. after 4 d at 28 °C.

Material examined: ITALY, Province of Forlì-Cesena, Teodorano - Meldola, on dead aerial branch of Vitis vinifera (Vitaceae), 21 February 2015, Erio Camporesi IT 2385 (MFLU 15-3509), culture MFLUCC 17-0961, CHINA, Beijing, on dead branch of V. vinifera, 3 June 2015, Ruvishika S. Jayawardena, culture JZB310176.

Notes: Botryosphaeria dothidea has been isolated from almost all grape growing regions in the world (Phillips et al. 2013, Dissanayake et al. 2016). According to the combined phylogenetic analysis of ITS and TEF gene regions, the isolates obtained from Vitis vinifera clustered with Botryosphaeria dothidea with a high bootstrap support.