Diplodia crataegicola
Diplodia crataegicola Dissan., Camporesi & K.D. Hyde, in Ariyawansa et al., Fungal Diversity: 10.1007/s13225-015-0346-5, [25] (2015)
Index Fungorum number: IF 551316; Facesofungi number: FoF 00885
Etymology: Referring to the host Crataegus sp.
Holotype: MFLU 15-1311.
Saprobic on dead branch of Crataegus sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 220–265 μm high × 260–380μm diam. (x̄ = 245 × 340 μm, n = 10), pycnidial, stromatic, solitary or clustered, immersed in the host, erumpent at maturity, dark brown to black, ostiolate, apapillate. Peridium 25–35 μm wide, outer and inner layers composed of dark brown and thin-walled hyaline textura angularis. Conidiogenous cells 10–22 μm high × 4–6 μm wide, hyaline, thin-walled, smooth, cylindrical, swollen at the base, discrete, producing a single conidium at the apex. Conidia 11– 16 × 6–10 μm (x̄ = 14 × 9 μm, n = 50), aseptate, globose to subglobose, widest in the center, with rounded apex, initially hyaline, becoming dark brown before release from the pycnidia, wall moderately thick, externally smooth, internally roughened. Spermatia rod-shaped with obtuse ends, hyaline, thin-walled, smooth, 3–5 × 1.5–2 μm.
Culture characteristics: Conidia germinating on WA within 12 h and germ tubes produced from lower end. Colonies growing on PDA, covering the entire plate in 5 days at 28 °C, mycelium grey to olivaceous black at the surface and olivaceous black from below.
Material examined: ITALY, Province of Forlì-Cesena[FC], Passo del Barbotto–Mercato Saraceno, on dead branch of Crataegus sp. (Rosaceae), 3 November 2012, E. Camporesi, IT 875 (MFLU 15-1311, holotype), ex-type living cultures, MFLUCC 15-0648, KUMCC15-0075, CFTCC 15-0002.
GenBank Numbers: ITS: KT290244, TEB2: KT290246, EF1-α: KT290248 .
Notes: Conidial length of all reported Diplodia species range from 21.5 to 52.5 μm and width vary from 10 to 22 μm (Phillips et al. 2013). The small conidia of Diplodia crataegicola (14 × 9 μm, L/W ratio = 1.55) clearly distinguish this species from all other reported species until 2015. Diplodia crataegicola is phylogenetically most closely related to D. seriata and the two species can be separated on the shapes and dimensions of their conidia.
Figure x. Diplodia crataegicola (holotype) a, b Conidiomata on host substrate. c, d Cross section of conidiomata. e, g Immature and mature conidia attached to conidiogenous cells. h Immature conidium. i–k Mature conidia l Germinating conidia. m Spermatogenous cells and spermatia. Scale bars: a = 2 mm, b = 500 μm, c = 100 μm, d = 75 μm, e, f = 50 μm, g–m = 15 μm 20 μm.
References:
Ariyawansa HA, Hyde KD, Jayasiri SC, Buyck B et al. 2015 – Fungal diversity notes 111–252-taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity: an international journal of mycology 75, 27–4.
Phillips AJL, Alves A, Abdollahzadeh J, Slippers B et al. 2013 The – Botryosphaeriaceae: genera and species known from culture. Studies in Mycology 76, 51–167.