Diplodia galiicola
Diplodia galiicola Dissan., Camporesi & K.D. Hyde, in Ariyawansa et al., Fungal Diversity: 10.1007/s13225-015-0346-5, [28] (2015)
Index Fungorum number: IF 551315; Facesofungi number: FoF 00884
Etymology: Referring to the host Galium sp.
Holotype: MFLU 15-1310.
Saprobic on stem of Galium sp. Sexual morph: Undetermined. Asexual morph: Conidiomata 320–385 μm high × 425–490 μm diam. (x̄ = 350 × 470μm, n = 10), stromatic, solitary, immersed in the host, dark brown to black, slightly depressed, uniloculate, globose to subglobose, ostiole central, apapillate. Peridium 25–35 μm wide, outer layer composed of dark brown cells of textura angularis, inner layers of thin-walled hyaline cells of textura angularis. Conidiogenous cells 8–15 μm high × 5–7 μm wide, hyaline, thin-walled, smooth, cylindrical, swollen at the base, discrete, producing a single conidium at the apex. Conidia 17–23 × 11–13 μm (x̄ = 20 × 12 μm, n = 50), initially hyaline, becoming dark brown, moderately thick-walled, wall externally smooth, roughened on the inner surface, aseptate, ovoid, widest in the center, apex obtuse, base truncate or rounded.
Culture characteristics: Conidia germinating on WA within 24 h and germ tubes produced from lower end. Colonies growing on PDA, covering the entire plate in 1 week at 28 °C, developing dense aerial mycelium with age, become pale olivaceous-grey to olivaceous black at the surface, and olivaceous black from below.
Material examined: ITALY, Province of Forlì-Cesena [FC], Strada San Zeno, Galeata, on dead stem of Galium sp. (Rubiaceae), 30 October 2013, E. Camporesi, IT 1495 (MFLU 15-1310, holotype), ex-type living cultures, MFLUCC 15-0647, KUMCC15-0074, CFTCC 15-0001.
GenBank Numbers: ITS: KT290245, TUB2: KT290247, EF1-α: KT290249.
Notes: In the combined phylogenetic analysis (ITS, EF1-α and β-tubulin genes), Diplodia galiicola is phylogenetically most closely related to D. seriata with high bootstrap support (1.0 BYPP). The conidia of D. galiicola differ from D. seriata in being shorter. The average conidia of D. seriata are longer than or equal to 25μm (Phillips et al. 2013), while the conidia of D. galiicola are never 25 μm long.
Figure x. Diplodia galiicola (holotype) a, b Conidiomata on host substrate. c Cross section of conidioma. d Mature and immature conidia with conidiogenous cells. e Immature conidia attached to conidiogenous cells. f Mature conidia attached to conidiogenous cells. g, h Mature and immature conidia. i, j Immature conidia. k, l Mature conidia. Scale bars: c = 200 μm, d–l = 20 μm.
References:
Ariyawansa HA, Hyde KD, Jayasiri SC, Buyck B et al. 2015 – Fungal diversity notes 111–252-taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity: an international journal of mycology 75, 27–4.
Phillips AJL, Alves A, Abdollahzadeh J, Slippers B et al. 2013 The – Botryosphaeriaceae: genera and species known from culture. Studies in Mycology 76, 51–167.