Botryosphaeriales » Botryosphaeriaceae » Diplodia

Diplodia seriata

Diplodia seriata De Not., Mém. R. Accad. Sci. Torino, Ser. 2 7: 26 (1845)

Index Fungorum number: 180468; Facesofungi number: FoF 03596

Holotype: ITALY, on dead stems of Jasminium sp., 18 August 1837, De Notaris (HERB RO).

Epitype: PORTUGAL, Montemor-o-Novo, on dead stems of Vitis vinifera, 31 July 1997, A.J.L. Phillips, CBS-H 19809, ex-epitype culture CBS 112555.

Pathogenic or Saprobic on dead branch of Rosa canina L. Sexual morph: Undetermined. Asexual morph: Conidiomata 220–265 μm high × 260–380 μm diam. (x̄ = 245 × 340 μm, n = 10), pycnidial, stromatic, solitary or clustered, immersed in the host, erumpent at maturity, dark brown to black, ostiolate, apapillate. Peridium 25–35 μm wide, outer and inner layers composed of dark brown and thin-walled hyaline textura angularis. Conidiogenous cells 10–22 μm high × 4–6 μm wide, hyaline, thin-walled, smooth, cylindrical, swollen at the base, discrete, producing a single conidium at the apex. Conidia 21–27 × 11– 16 μm (x̄ = 24 × 13 μm, n = 50), globose to subglobose, with rounded apex, initially hyaline, becoming dark brown, wall moderately thick.

Culture characteristics: Conidia germinating on WA within 12 h and germ tubes produced from both ends. Colonies growing on PDA, covering the entire plate in 5 days at 28 °C, mycelium grey to olivaceous black at the surface and olivaceous black from below.

Material examined: ITALY, Province of Forlı`-Cesena [FC], Passo del Barbotto, Mercato Saraceno, on dead aerial branch and spines of Rosa canina L. (Rosaceae), 29 November 2014, Erio Camporesi, IT 2254 (MFLU 15-1311), living culture MFLUCC 17-0954.

GenBank Numbers: ITS: MG828896, TEF: MG829266.

Note: During our investigation on the diversity of microfungi in Italy, an isolate (MFLUCC 17–0954) was recovered from Rosa spines in Forli-Cesena Province. This new isolate shares a close phylogenetic affinity to Diplodia seriata (CBS 112555) in our combined ITS and TEF sequenced data analyses. This relationship is only supported in our ML analysis, whereas other analyses yielded no support. However, nucleotides of the ITS and TEF regions are identical and we therefore introduce MFLUCC 170954 as another strain for Diplodia seriata. Diplodia rosae, D. rosarum, D. spurca have also been reported from Rosa in Bulgaria, California, Cuba, Greece, Pakistan, Spain and Ukraine (Farr and Rossman 2017) although these records are not backed up with molecular data. This is the first record of Diplodia seriata on Rosa.