Dothiorella iberica
Dothiorella iberica A.J.L. Phillips, J. Luque & A. Alves, Mycologia 97(2): 524 (2005)
Index Fungorum number: IF344530; Facesofungi number: FoF 03513
Saprobic on branches of Rosa canina L. Sexual morph: Undetermined. Asexual morph: coelomycetous. Conidiomata 150–275 μm high × 200–325 μm diam. (x̄ = 225 × 275 μm, n = 10), pycnidial, solitary or aggregated, semi-immersed to immersed, dark brown to black, slightly depressed, globose to subglobose, unilocular, ostiolate central. Peridium 10–25 μm wide, composed of 3–5-layers of brown to black-walled cells of textura angularis. with outer 1–3-layers brown and inner 1–2-layers hyaline, thin-walled cells of textura angularis. Conidia 22–28 × 11–13 μm (x̄ = 24 × 12, n = 20), subglobose to ellipsoidal, pale brown when young, becoming brown at maturity, smooth thick-walled, one median septate.
Culture characteristics: Colonies on PDA, covering entire petri dishes after 12 d in the dark at 25 ºC; circular, initially white, after 7 d becoming greyish brown to black; reverse grey to dark greyish green.
Material examined: ITALY, Province of Forlı`-Cesena [FC], near Strada San Zeno, Galeata, on a dead aerial branch of Rosa canina (Rosaceae), 17 October 2014, Erio Camporesi IT 2179 (MFLU 17–0290), living culture MFLUCC 17–0951.
GenBank numbers: ITS: MG828897, TEF: MG829267
Note: An isolate (MFLUCC 17–0951) was recovered from Rosa stems from Forli-Cesena Province. This new isolate shares a close phylogenetic affinity to Dothiorella iberica (CBS 115045) in our combined ITS and TEF sequenced data analyses. This species has not been reported from the plant family Rosaceae and here we provide the first association of this species with Rosa.
Figure 1. Dothiorella iberica (MFLU 17–0290). a Conidiomata on host. b Cross-section of the conidiomata. c–d Immature and mature conidia with conidiogenous cells. e Immature pale-yellow conidia with conidiogenous cells. f Mature, dark brown conidia. g–h Mature dark brown conidia. Scale bars: b = 100 μm, c–f = 30 μm, g–h = 20 μm.
References:
Wanasinghe DN, Phukhamsakda C, Hyde KD, Jeewon R et al. 2018 – Fungal diversity notes 709–839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal diversity 89, 1–236.
Other Collections
Dothiorella iberica A.J.L. Phillips, J. Luque & A. Alves, Mycologia 97(2): 524 (2005)
Index Fungorum number: IF344530; Facesofungi number: FoF 03513
Saprotrophic on dead and aerial branches of Vitis vinifera. Sexual morph: Not observed. Asexual morph: Conidiomata up to 250 μm high, 400 μm diam., pycnidial, stromatic, solitary, globose, thick-walled, composed of dark brown thick-walled textura angularis, becoming thin-walled and hyaline towards the inner region. Conidiophores absent. Conidiogenous cells 8–15 × 3–5 μm, lining the pycnidial cavity, holoblastic, hyaline, subcylindrical. Conidia 19–24 × 9–10 μm (x̄ =22 × 9 μm, n=30), initially hyaline, becoming dark brown and one-euseptate, often while still attached to the conidiogenous cell, ovoid with a broadly rounded apex and truncate base, brown walled, 1-septate, slightly constricted at the septum.
Culture characteristics: Colonies initially white to olivaceous buff, becoming greenish olivaceous from the middle of colonies within 7 days, iron-grey (surface) and black (reverse) with age, with suppressed, moderately fluffy mycelium, covering 90 mm diam Petri dishes after 4 days in the dark.
Material examined: ITALY, Province of Forlì-Cesena, Tessello - Cesena, on dead and aerial branch of Vitis vinifera (Vitaceae), 5 March 2015, Erio Camporesi IT 2404 (MFLU 15-3513), culture, MFLUCC 17-0964.
Notes: Dothiorella iberica has been widely isolated from Vitis vinifera world-wide and is a causal agent of Botryosphaeria die back (Phillips et al. 2013). This study provides the first report of D. iberica as a saprotroph on Vitis vinifera from Italy (Jayawadena et al. 2018).
Figure 2. Dothiorella iberica. a, b. Conidiomata immersed on host surface. c. Longitudinal section of a conidioma. d, e. Conidiogenous cells with developing conidia. f. Immature conidium. g, h. Mature conidia. i. Germinating conidium. Scale bars: c=100 μm, d–i=20 μm.
Dothiorella iberica A.J.L. Phillips, J. Luque & A. Alves 2005
Index Fungorum number: IF 344530; Facesoffungi number: FoF 03513
Saprobic on a dead aerial branch of Quercus ilex L. Sexual morph: Ascomata 250–350 μm diam., 275–400 μm high (x̄ = 275 × 300 μm, n = 5), pseudothecial, globose, scattered on the substrate, semi-immersed, erumpent at maturity, ostiolate, black. Ostioles 60–70 μm high, circular, papillate, central, single. Peridium 40–55 μm thick, several layers, from outer to inside dark brown to hyaline cells of textura angularis. Hamathecium comprises 2–3 μm wide, thin-walled, septate, hyaline numerous pseudoparaphyses. Asci 60–140 × 12–22 μm (x̄ = 95 × 15 μm, n = 10), stipitate, arising from the base of the ascoma, cylindric-clavate, bitunicate, with a well-developed apical chamber, 8-spored, uniseriate, biseriate or multiseriate. Ascospores 19–25 × 7–12 μm (x̄ = 22 × 9 μm, n = 10; l/w = 2.4), elliptical, widest in the middle part, aseptate to 1-septate, slightly constricted at the septum, tapering towards the base, moderately thick-walled, finely verruculose on the inner surface, initially hyaline, becoming dark brown at maturity. Asexual morph: see Phillips et al. (2013).
Material examined: Italy, Province of Forlì-Cesena [FC]), near Colmano, on a dead aerial branch of Quercus ilex L. (Fagaceae), 27 November 2018, Erio Camporesi, IT 4127b (MFLU 19-0302).
GenBank Accession Numbers: ITS: OQ401061; LSU: OQ411146; tub2: OQ437900.
Notes: Italian records of D. iberica (asexual morph) were found on Acer opalus (Sapindaceae), Rosa canina (Rosaceae), and Vitis vinifera (Vitaceae) (Dissanayake et al. 2016a, Jawawardena et al. 2018c, Wanasinghe et al. 2018). This collection was found in the same locality but on a different host species, Quercus ilex (Fagaceae). Therefore, this is the first record of D. iberica on Italian Q. ilex trees, and the first sexual morph of D. iberica reported from Italian collections (Wijesinghe et al. 2023).
Figure x. Dothiorella iberica (MFLU 19-0302, new host record in Italy). a–b Ascomata on the dead host surface of Quercus ilex L. (Fagaceae, Fagales). c–d Sections of ascomata. e Peridium. f Pseudoparaphyses. g–k Asci. l–p Ascospores. Scale bars: a, b = 500 μm, c = 200 μm, d = 100 μm, e–k = 10 μm, l–p = 5 μm.
N.B - The data has been directly extracted from the original publication (Wijesinghe et al. 2023) with the permission of the journal Current Research in Environmental & Applied Mycology' (Journal of Fungal Biology).
References
Jayawardena RS, Hyde KD, Chethana KW, Daranagama DA et al. 2018 – Saprotrophic fungi on Vitis in China, Italy, Russia and Thailand. Mycosphere notes 9, 1–114.
Phillips AJL, Alves A, Abdollahzadeh J, Slippers B et al. 2013 – The Botrysphaeriaceae: genera and species known from culture. Studies in Mycology 76, 51–167.
Wijesinghe SN, Samarakoon MC, Camporesi E, Hyde KD et al. 2023 – Over the footprints of Italian mycology with emphasis on plant-associated Ascomycota. Current Research in Environmental & Applied Mycology (Journal of Fungal Biology), 13, 162–276.
Last Update: 25 October 2023