Dothiorella sarmentorum
Dothiorella sarmentorum (Fr.) A.J.L. Phillips, A. Alves & J. Luque, Mycologia 97(2): 522 (2005)
Index Fungorum number: IF 501403; Facesofungi number: FoF 00171
Saprotrophic on dead and aerial branches and leaves of Vitis vinifera. Sexual morph: Not observed (see Phillips et al. 2013 for a description). Asexual morph: Conidiomata 300–440 μm high × 215–290 μm diam. (x̄ = 376 × 250 μm, n = 5), stromatic, solitary or scattered in small groups, immersed, uni-loculate, individual or aggregated, black, globose to subglobose, ostiolate. Conidiomatal wall comprising several layers; outer layers composed of thick-walled, dark brown, somewhat flattened cells of textura angularis and inner layers of larger, thin-walled, lightly pigmented or hyaline cells. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 7–15 × 3–5 μm (x̄ = 10 × 3 μm, n=15), lining the conidiomatal cavity, holoblastic, hyaline, subcylindrical, proliferating at the same level giving rise to periclinal thickenings. Conidia 18–23×8–10 μm (x̄ = 20 × 9 μm, n=30), ovoid, with a broadly rounded apex and truncate base, initially hyaline to lightly pigmented and aseptate, becoming dark brown and 1-septate, slightly constricted at the septum, smooth-walled
Culture characteristics: Colonies on PDA at 25° C attaining 80 mm diam in 5 days, circular, flat, moderately dense, surface initially white, becoming greenish olivaceous to greyish within 7 days, smooth surface with entire to slightly undulate edge.
Material examined: ITALY, Province of Forlì-Cesena, near Pieve di Rivoschio, on dead and aerial branch of Vitis vinifera (Vitaceae), 2 February 2015, Erio Camporesi IT 2363 (MFLU 15-1082); culture, MFLUCC 17-1212, 17-1213, 17-1253; ITALY, Province of Forlì-Cesena, Teodorano - Meldola, on dead and aerial branch of Vitis vinifera (Vitaceae), 21 February 2015, Erio Camporesi IT 2386 (MFLU 15-3510); culture, MFLUCC 17-0962; CHINA, Beijing, on dead attached leaf of Vitis vinifera (Vitaceae), 3 June 2015, Ruvishika S. Jayawardena, culture JZB3150039.
Notes: According to the combined phylogenetic analysis of ITS and TEF gene regions, the isolates obtained from V. vinifera in this study are recognized as D. sarmentorum. Dothiorella sarmentorum has a world-wide distribution (Dissanayake et al. 2016) and reported to be a major pathogen in grapevine trunk diseases (Pitt et al. 2013).
Figure 1. Dothiorella sarmentorum. a. Conidiomata immersed on host surface. b. Section of a conidioma. c. Conidiogenous cells with developing conidia. d. Mature conidia. Scale bars: b=100 μm, c–d=20 μm.
References:
Jayawardena RS, Hyde KD, Chethana KW, Daranagama DA et al. 2018 – Saprotrophic fungi on Vitis in China, Italy, Russia and Thailand. Mycosphere notes 9, 1–114.
Phillips AJL, Alves A, Abdollahzadeh J, Slippers B et al. 2013 – The Botrysphaeriaceae: genera and species known from culture. Studies in Mycology 76, 51–167.
Dissanayake AJ, Philips AJL, Li XH, Hyde KD. 2016 – Botryosphaeriaceae: Current status of genera and species. Mycosphere 7, 1001–1073.
Pitt WM, Úrbez-Torres JR, Trouillas FP. 2013 – Dothiorella vidmadera, a novel species from
grapevines in Australia and notes on Spencermartinsia. Fungal Diversity 61, 209–219.
Last Update: 02 September 2021