Diaporthe rudis
Diaporthe rudis (Fr.) Nitschke, Pyrenomyc. Germ. 2: 282 (1870)
Index Fungorum number: IF 139900; Facesofungi number: FoF 02353
Saprobic on dead branches of Rosa canina L. Sexual morph: Ascomata 200–300 μm high 230–320 μm diam. (x̄ = 220 × 227 μm, n = 5, immersed in the ectostroma, erumpent through the host epidermis, visible as raised, pale yellow-brown patches on the host surface, globose to flattened globose, solitary or occur in clusters, black-brown, with a neck. Neck 205 × 99 diam. Ostiole periphysate. Peridium 25–40 μm wide, comprising 10–15 layers, outer layers heavily pigmented, thin-walled, comprising dark brown cells of textura globulosa to textura angularis, inner layers composed of hyaline to brown thin-walled cells of textura angularis. Paraphyses 6–9 μm (n = 10) 1–4-septate, wide at base, tapering towards the apex, thin-walled. Asci 40–50 x̄ 7–12 μm (x̄ = 47.8 × 9.1 μm, n = 20), 8-spored, unitunicate, cylindrical to cylindric clavate, straight to slightly curved, sessile, with a J- apical ring. Ascospores 9–12 × 2.5–3.5 μm (x̄ = 11.3 × 3.2 μm, n = 30), overlapping uniseriate to biseriate, 1-septate, often tetra-guttulate, with larger guttules at center and smaller ones at the ends, ellipsoidal to clavate, straight to slightly curved, hyaline, without appendages or a mucilaginous sheath. Asexual morph: See Udayanga et al. (2014).
Material examined: ITALY, Forlı`-Cesena Province, near Corniolo, Santa Sofia, dead aerial branches of Rosa canina L. (Rosaceae), 20 September 2014, Camporesi Erio, IT 2111 (MFLU 15-3379, ex-type living cultures = MFLUCC 14-1209).
GenBank: TUB: KX856059, ITS: KX856060, LSU: KX856061, SSU: KX856062, TEF: KX856063.
Notes: Our new isolate MFLUCC 14-1209 clustered with ex-type strain of Diaporthe rudis (AR 3422), which was originally described by Nitschke (1870) from Laburnum anagyroides (as Cytisus laburnum), but is also recorded from Rosa in Australia (Udayanga et al. 2014). However, it differs from the type strain of D. rudis (Nitschke 1870, Udayanga et al. 2014) in having smaller asci (44–53 × 7.2–11.2 μm versus 53.5–58.5 × 10.6–12 μm) and ascospores (9.4–12.3 × 2.7–3.5 μm versus 12–14.2 × 3.5–3.7 μm). By considering the molecular data and the host, we consider our collection as a new record from Rosa canina L. in Italy.
Figure x. Diaporthe rudis (MFLU 15-3379) a, b Appearance of ectostroma and ascomata on host substrate c Vertical section through ectostroma with ascoma. d Section through ostiole. e Close up of peridium. f Paraphyses. g–i Immature and mature asci. j Ascus in Melzer’s reagent. k Germinating ascospore. l, m Ascospores. Scale bars: b = 1 mm, c–j = 20 μm, k–m =5 μm.
References:
Nitschke T 1870 – Pyrenomycetes Germanici. Breslau, Eduard Trewendt, Germany 2:161–320
Udayanga D, Castlebury LA, Rossman AY, Hyde KD 2014 – Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporella, D. foeniculina and D. rudis. Persoonia: Molecular Phylogeny and Evolution of Fungi. 32, 83–101.
Wanasinghe DN, Phukhamsakda C, Hyde KD, Jeewon R et al. 2018 – Fungal diversity notes 709–839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal diversity 89, 1–236.
Last update: 30 November 2021