Hapalocystis berkeleyi Auersw. ex Fuckel, Fungi rhenani exsic., fasc. 6: no. 585 (1863)
Index Fungorum number: IF 357284; Facesofungi number: FoF 00413
Reference specimen: MFLU14–0798
Saprobic on bark of Platanus hybrid. Sexual morph: Ascomata 90–185 μm high × 206–302 μm wide (x̄ =150 × 245 μm, n = 15), immersed, solitary or aggregated, scattered, visible through dark cracks on the host surface, subglobose to globose, ostiolate. Ostiole 130–195 μm high × 90–113 μm wide (x̄ = 145 × 105 μm, n = 10), short papillate, black, cylindrical, periphysate. Periphyses hyaline. Peridium 4–16 μm wide (x̄ = 10 μm, n = 15) black, thick-walled, 3–5-layered of cells of textura angularis. Paraphyses 3–7μm wide, few, septate, hyaline, attached to the base, longer than asci. Asci 112–127 × 46–80 μm (x̄ = 121 × 66 μm, n = 20) 4 or 8-spored, unitunicate, clavate to fusoid, with a short pedicel, with an inconspicuous flat, refractive ring at the lower end of the thickened apical wall, apex narrow and blunted. Ascospores 34–42 × 12–18 μm (x̄ = 37 × 17 μm) inequilaterally ellipsoidal,withbroadlyrounded ends, initially hyaline, dark brown at maturity, (1–)2(–3)- septate, with cells of equal length, thick and smooth-walled, with short, hyaline strap-like appendages situated at both rounded ends with same width as the ascospore. Asexual morph: Conidiomata on MEA 165–195 μm diam. (x̄ = 182 μm, n = 15), pycnidia, superficial, aggregated, 3–5 in one group, globose, orange to brown, Conidiomatal wall 7–13 μm wide (x̄ =10 μm, n = 10) small,thick-walled,orange, 5–10cells layers of textura angularis. Conidiophores 1.5–2 μm high × 1– 1.5 μm wide (x̄ = 2 × 1.5 μm, n = 10) branched, hyaline, short, few conidiogenous cells arising from one conidiophore, attached to conidiomatal wall. Conidiogenous cells 10–14 μm long, 5–7 μm wide, cylindrical, hyaline, bottle-shaped, septate, ends pointed, phialidic. Conidia 0.5–1.5 diam. (x̄ = 1 μm, n = 20) ellipsoid, one-celled, hyaline, smooth-walled.
Culture characteristics: Ascospores germinating on MEA after 2daysat16°Cforminghyaline, branched germ tubes form end cells. Colonies on MEA reaching 2 cm after 14 days at 25 °C in light, greenish ash, effuse, circular, marginentire, surface rough.
Material examined: ITALY,ProvinceofForlì-Cesena[FC], Modigliana, Montebello, on branch of Platanus hybrid, 14 April 2013, E. Camporesi It 1187 (MFLU 14-0798, reference specimen of Hapalocystis berkeleyi designated here); extype living cultures, MFLUCC 13-0662.
GenBank Numbers: LSU: KP744486.
Notes: Hapalocystis (Sydowiellaceae, Diaporthales) is typified by H. berkeleyi. LSU gene analysis of diaporthalean fungi showed Hapalocystis as a distinct genus in Sydowiellaceae (Castlebury et al. 2002). Jaklitsch and Voglmayr (2004) evaluated Hapalocystis introducing a new species. Hapalocystis comprises H. occidentalis, H. ulmi, H. bicaudata, H. corticalis and H. berkeleyi (H. berkeleyi Auersw. ex Fuckel var. berkeleyi and H. berkeleyi var. kickxii (Westend.) M.E. Barr (Jaklitsch and Voglmayr 2004). The asexual morph of Hapalocystis is given as Stilbospora, Hendersonia or Dothiorella (Wehmeyer 1941), or as stilbospora-like (Barr 1978). Glawe (1985) reported a phoma-like asexual morph for H. berkeleyi in culture. In this study we observed a phoma-like asexual morph on MEA and we made fresh collections of Hapalocystis berkeleyi which is identical to the morphology of type specimen. The type specimen is in good condition (at herbarium K) and hence, we designate this as a reference specimen (Ariyawansa et al. 2014) and provide molecular data; the asexual morph was also produced in culture.