Dothiora buxi Jayasiri, Camporesi & K.D. Hyde, in Hyde et al., Fungal Diversity 80: 30 (2016)
Index Fungorum number: IF 552173; Facesofungi number: FoF 02223
Etymology: The specific epithet buxi is based on the host genus from which the taxon was collected.
Holotype: MFLU 15-3404.
Saprobic on Buxus sempervirens L. Sexual morph: Ascostromata 500–1000 μm long × 220–250 μm high, 320–340 μm diam., erumpent through the epidermis, solitary or clustered, globose, brown to black, with single locules, with a central longitudinal slit-like opening. Peridium 32–83 μm wide, two-layered, outer layer composed of dark brown or brown, thick-walled cells of textura angularis, inner layer composed of hyaline, thin-walled cells of textura angularis. Hamathecium lacking pseudoparaphyses. Asci 100–115 × 14–21 μm (x̄ = 102 × 17 μm, n = 20), 32-spored, bitunicate, fissitunicate, cylindro-clavate, short pedicellate, apically rounded, with a small ocular chamber. Ascospores 11–15 × 5.4–7 μm (x̄ = 13 × 6 lm, n = 30), bi-seriate to multi-seriate, hyaline to very pale brown, aseptate, fusoid to ovoid, one end narrower than other, smooth-walled with granular contents, with a thin mucilaginous sheath.
Material examined: ITALY, Province of Forlı`-Cesena [FC]), near Passo delle Forche—Galeata on dead branch of Buxus sempervirens (Buxaceae), 17 November 2014, E. Camporesi, IT 2284 (MFLU 15-3404, holotype, KUN, isotype).
Notes: Dothiora was introduced by Fries (1849) with D. pyrenophora (Fr.) Fr. as the type species. Our isolate shares common characters with the genus Dothiora. DNA was extracted from fruiting bodies and multi-gene phylogenetic analysis placed Dothiora buxi as a sister taxon to Dothiora elliptica. The latter is similar in having epidermal erumpent, hysteriiforme ascostromata and hyaline ascospores but differs from Dothiora buxi in having 32-spored asci and aseptate, fusoid to ovoid, ascospores narrowed at one end (Saccardo 1889). Therefore, we introduce Dothiora buxi as a new species (Hyde et al. 2016).