Heterosphaeria patella
Heterosphaeria patella (Tode) Grev., Scott. crypt. fl. (Edinburgh) 2: 103 (1823)
Index Fungorum number: IF186470; Facesofungi number: FoF 07095
≡ Sphaeria penetrans a patella Tode, Fung. mecklenb. sel. (Lüneburg) 2: 45 (1790)
Neotype: GERMANY, Altburg, Regensberg on a Daucus carota, 7 August 1987, leg. A.L. Leuchtmann 9634 (ZT 9634).
Saprobic on dead stem. Sexual morph: Apothecia 275–923 × 238–656 μm (x̄ = 585 × 487.5 μm, n = 10), when dry arising solitary, scattered on wood, sessile, superficial, deep cupulate, black, rough surface. Receptacle concave. Disc flat, black. Margin slightly curved, black. Hymenium inner mixed with asci and paraphyses, hyaline. Subhymenium forming a hyaline, small-celled tissue. Ectal excipulum 34.5–49 μm (x̄ = 41.5 μm, n = 10), composed of hyaline cells of textura globulosa, 4–6 layers, thin-walled cells. Medullary excipulum 58–157 μm (x̄ = 90 μm, n = 10), composed with hyaline cells of textura intricata, thick-walled. Paraphyses 1–2 μm (x̄ = 1.5 μm, n = 20), long-thin form, filiform, 1–3-septate, thin-walled, hyaline, swollen at the apex. Asci 55–65 × 6.5–9 μm (x̄ = 59 × 7.5, n = 20), 8-spored, unitunicate, inoperculate, cylindric-clavate, trapezoidal apex, thinwalled, blunt end, J +, ring. Ascospores 10–12 × 3–5 μm (x̄ = 10.5 × 4 μm, n = 10), 1-seriate overlapping, arranged in a whole length of asci, fusiform, ellipsoidal, hyaline, thin-walled, large oil drop at both ends, aseptate or 1-septate, smooth-walled. Asexual morph: Undetermined.
Material examined: ITALY, Forlì-Cesena (FC), Corniolo, Santa Sofia, on dying stems of Anthriscus cerefolium (Apiaceae), 20 July 2018, E. Camporesi, IT 3978 (MFLU 18–1491)
GenBank Numbers: ITS: MN860230, LSU: MN860231.
Note: The phylogenetic results from different analysis (ML, MP and BI) show the same topology of Heterosphaeria patella strain MFLU 18–1491 that grouped as sister to H. patella (G.M. 2014–08–04–1) with low support in ML and MP analyses (51% ML, 58% MP) but strong support in BI analysis (0.99 BYPP). Heterosphaeria patella strain MFLU 18–1491 was found on Anthriscus cerefolium. This is the first report on this host. The morphology of our strain is very similar to H. patella in having ectal excipulum of cells of textura globulosa, 1–3-septate on paraphyses swollen at the apex, cylindric-clavate with J + apical ring and ellipsoidal, aseptate or 1-septate ascospores with large guttules at both ends. There are some different morphological features between our collection and H. patella (Leuchtmann 1987). Our strain has a medullary excipulum of cells of textura intricata, while H. patella (Leuchtmann 1987) has textura porrecta. Heterosphaeria patella (MFLU 18–1491) is 100% identical with H. patella (G.M. 2014–08–04–1) both in LSU and ITS. Therefore, we report a new host record and update sequence data for this species.
Figure x. Heterosphaeria patella (MFLU 18–1491). a Apothecia on wood. b Close up of apothecia. c Vertical cross section. d Part of a cross section. e Filiform paraphyses. f Cylindrical asci in Melzer’s reagent. g–i Cylindrical asci. j J + , apical ring in Melzer’s reagent. k–l Ellipsoidal ascospores. Scale bars: a = 2000 μm, b = 100 μm, c = 50 μm, d = 20 μm e, j = 10 μm, f–i = 5 μm, k–l = 3 μm.
References:
Hyde KD, Dong Y, Phookamsak R, Jeewon R et al. 2020 – Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 16, 1–273.
Leuchtmann A 1987 – Species of Heterospaeria (Discomycetes) and their anamorphs. Mycotaxon 28, 261–284.