Pseudomassariosphaeria bromicola
Pseudomassariosphaeria bromicola Phukhams., Ariyaw., Camporesi & K.D. Hyde, in Ariyawansa et al., Fungal Diversity: 10.1007/s13225-015-0346-5, [14] (2015)
Index Fungorum number: IF 551368; Facesofungi number: FoF 00917
Holotype: MFLU 15–1403.
Saprobic on dead stem of Bromus sterilis. Sexual morph: Ascomata 205–249 μm high × 194–223 μm diam. (x̄ = 233 × 208 μm, n = 10), on the surface of the host, solitary, scattered or gregarious, globose, erumpent or rarely superficial, black to dark brown, smooth, papillate, ostiolate with periphysoids. Peridium comprising two strata, outer stratum composed of 3–5 layers of dark brown to light brown cells of textura angularis, inner stratum comprising with 1–2 layers of hyaline flattened cells. Hamathecium of dense, 1.2–2.6 μm (x̄ = 2 μm, n = 20), broad, filiform, anastomosing, hyaline, transversely septate, long, cellular psedoparaphyses, embedded in a gelatinous matrix. Asci 82–115 × 12–19 μm (x̄ = 101 × 15 μm, n = 20), (7-)8-spored, bitunicate, fisitunicate, cylindrical to clavate, short pedicellate, apically round with ocular chamber up to 1–2 μm wide, 1–2 μm high. Ascospores 21–36 × 4–9 μm (x̄ = 31 × 7 μm, n = 50), bi-seriate or overlapping, hyaline, fusiform to lunate, narrow towards the apex, 8–9-septate, constricted at septum, granulate, surrounded with wide mucilaginous sheath, 5–7 μm wide at apex and base, and 10–12 μm wide at sides. Asexual morph: Undetermined.
Culture characteristics: Colonies on PDA, reaching 4 cm diam. after 4 weeks at 16 °C, with dense mycelia, circular, rough margin white, becoming dark olive brown (4F8) after 2 weeks, flat on the surface, without aerial mycelium, producing black oil droplets after 4 weeks, lobate at lower margin, dark brown to black. Hyphae septate branched, containing small granules, brown.
Material examined: ITALY, Province of Forlì-Cesena, Fiumicello-Premilcuore, on dead stem of Bromus sterilis (Poaceae), 15 June 2013, E. Camporesi (MFLU 15–1403, holotype), (isotype in HKAS, under the code of HKAS88969), ex-type living culture, MFLUCC 15–0031.
GenBank Numbers: ITS: KT305998, LSU: KT305994, SSU: KT305996, EF1-α: KT305999.
Figure x. Pseudomassariosphaeria bromicola (holotype) a Ascomata on host surface. b Section of ascomata. c Section of partial peridium layer. The peridium comprising textura angularis. d Hyaline pseudoparaphyses. e Immature asci. f, g. Mature asci with short pedicels h–k. Hyaline ascospores with visible mucilaginous sheath. l Ascospore stained with Indian ink to show sheath. m Germinating ascospore. n, o Colonies on PDA from surface and reverse. Scale bar: a = 200 μm, b = 100 μm, c = 50 μm, d–g = 50 μm, e–g = 20 μm, h–k = 10 μm, l–m = 20 μm.
References:
Ariyawansa HA, Hyde KD, Jayasiri SC, Buyck B et al. 2015 – Fungal diversity notes 111–252-taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity: an international journal of mycology 75, 27–4.