Pleosporales » Amorosiaceae » Angustimassarina

Angustimassarina coryli

Angustimassarina coryli Wanas., Camporesi, E.B.G. Jones & K.D. Hyde, in Hyde et al., Fungal Diversity 87: 9 (2017)

Index Fungorum number: IF 553259; Facesofungi number: FoF 03390

Etymology: referring to the host genus Corylus.

Holotype: MFLU 16-0218.

Saprobic on dead herbaceous branches of Corylus avellana L. Sexual morph: Ascostromata 150–250 μm high × 500–750 μm diameter (x̄ = 186.6 × 600.4 μm, n = 10), solitary, scattered, or in small groups of 2–3, superficial, with basal wall remaining immersed in host tissue, wall black, roughened, containing multi-locules, each locule 180–250 μm diameter, ostiolate. Peridium 15–25 μm thick, 1-layered, composed of small, heavily pigmented, thick-walled, cells of textura angularis, cell wall 10–20 μm thick between the locules. Hamathecium comprising numerous, 2–2.5 μm (n = 30) wide, filamentous, branched, septate, pseudoparaphyses, embedded in a gelatinous matrix. Asci 70–100 × 10–15 μm (x̄ = 85.7 × 12.8 μm, n = 40), 8-spored, bitunicate, fissitunicate, cylindrical to narrowly fusoid, pedicellate, thick-walled at the apex, with minute ocular chamber. Ascospores 20–25 × 5–8 μm (x̄ = 22.4 × 6.6 μm, n = 50), overlapping 1–2-seriate, broadly fusoid to fusoid, with broadly to narrowly rounded ends, hyaline, 1-septate, deeply constricted at the median septum, the upper cell often broader than the lower one, smooth-walled, containing four refractive globules, surrounded by an irregular hyaline gelatinous sheath. Asexual morph: Undetermined.

            Culture characteristics: Colonies on PDA reaching 20–25 mm diameter in 21 days, surface greenish-brown, with sparse to moderate aerial mycelium and feathery margins; reverse iron-grey.

            Material examined: ITALY, Province of Trento, Commezzadura, Malga di Almazzago, dead upright stems of Corylus avellana (Betulaceae), 11 August 2014, E. Camporesi, IT2052 (MFLU 16-0218, holotype), ex-type living cultures MFLUCC 14-0981, ICMP

GenBank numbers ITS: MF167431, LSU: MF167432, TEF1: MF167433

        Notes: Angustimassarina coryli resembles other Angustimassarina species in having an ostiole with a crest-like opening, the peridium with rounded dark pigmented cells of textura angularis, cylindrical to cylindric-clavate, short pedicellate asci and hyaline, fusiform ascospores containing large guttules. Angustimassarina coryli however, differs from the remaining species of Angustimassarina in having ascostromata with multi-locules, while others have uniloculate ascomata. Our phylogeny, albeit with weak support, supports our species as new and indicates a close relationship to A. italica and A. arevina (Hyde et al. 2017).