Pleosporales » Amorosiaceae » Angustimassarina

Angustimassarina populi

Angustimassarina populi Thambug. & K.D. Hyde, in Thambugala et al., Fungal Diversity 74:199–266 (2015)

Index Fungorum number: IF551279; Facesofungi number: FoF 01086

Etymology: Referring to the host genus Populus.

Holotype: MFLU 14-0588

            Fungicolous or saprobic on other fungi or Populus sp. in terrestrial habitats. Sexual morph: Ascomata 125–175 μm high, 100–120 μm (x̄ = 154 × 137.8 μm, n = 10) diam., solitary or gregarious, immersed, becoming erumpent, coriaceous, black, globose to subglobose, uniloculate, ostiolate. Ostiole crest-like, rounded, papillate, immersed in other ascomata, with a pore-like opening. Peridium 14–32 μm (x̄ = 23 μm, n = 15) wide, composed dark brown to hyaline cells of textura angularis. Hamathecium comprising 1–2 μm wide, septate, unbranched, cellular pseudoparaphyses, embedded in a gelatinous matrix. Asci 80–95 × 9.5–13 μm (x̄ = 87 × 11.4 μm, n = 15), 8-spored, bitunicate, fissitunicate, cylindrical, pedicellate, rounded at the apex, with an ocular chamber. Ascospores 19–22 × 3.2–5.5 μm (x̄ = 20.5 × 4.3 μm, n = 20), uni to bi-seriate, partially overlapping, hyaline, fusiform, 1(–3)-septate, constricted at the central septum, filled with a different sized guttule per cell and surrounded by a mucilaginous sheath. Asexual morph: hyphomycetous. Colonies on PDA moderately growing, circular with a wavy margin, cottony on the surface, grayish brown, with reverse dark brown. Conidiophores 2.5–4 μm wide, micronematous to semi-macronematous, arising singly and not in sporodochia or synemmata, pale brown, unbranched, similar to the mycelial hypha. Conidiogenous cells 4.2–9.8 × 2.3–3.4 μm (x̄ = 6.9 × 2.9μm, n = 10), integrated, terminal, holoblastic, short-cylindrical to elongate-cylindrical, brown to pale brown smooth-walled. Conidia 6.6–10 × 5.6–7.5 μm (x̄ = 8.3 × 6.5 μm, n = 10) solitary, dry, elongate-clavate, pale to dark brown, 1–3-septate, evenly pigmented, constricted at the septa, smooth-walled.

Culture characteristics: Ascospores germinating on PDA within 18 h and producing germ tubes from both ends. Colonies on PDA attaining 40 mm diam. after 5 weeks at 25 °C, flat, grayish-brown, with reverse dark brown, with a crenate edge. After 2 weeks the culture produces lightly pigmented chemical exudate. Mycelium well-developed, superficial, composed of pale brown, thin-walled hyphae, frequently septate, smooth-walled. Chlamydospores sometimes formed individually, pale brown, broadly globose to subglobose.

Material examined: ITALY, Province of Forlì-Cesena [FC], Strada San Zeno, Galeata, on dead branches of Populus sp. (Salicaceae), 15 November 2012, Erio Camporesi IT 896 (MFLU 14-0588, holotype); ibid., (HHUF 30460, isotype); ex-type living culture, MFLUCC 13-0034, ICMP 20742.