Angustimassarina populi
Angustimassarina populi Thambug. & K.D. Hyde, in Thambugala et al., Fungal Diversity 74:199–266 (2015)
Index Fungorum number: IF551279; Facesofungi number: FoF 01086
Etymology: Referring to the host genus Populus.
Holotype: MFLU 14-0588
Fungicolous or saprobic on other fungi or Populus sp. in terrestrial habitats. Sexual morph: Ascomata 125–175 μm high, 100–120 μm (x̄ = 154 × 137.8 μm, n = 10) diam., solitary or gregarious, immersed, becoming erumpent, coriaceous, black, globose to subglobose, uniloculate, ostiolate. Ostiole crest-like, rounded, papillate, immersed in other ascomata, with a pore-like opening. Peridium 14–32 μm (x̄ = 23 μm, n = 15) wide, composed dark brown to hyaline cells of textura angularis. Hamathecium comprising 1–2 μm wide, septate, unbranched, cellular pseudoparaphyses, embedded in a gelatinous matrix. Asci 80–95 × 9.5–13 μm (x̄ = 87 × 11.4 μm, n = 15), 8-spored, bitunicate, fissitunicate, cylindrical, pedicellate, rounded at the apex, with an ocular chamber. Ascospores 19–22 × 3.2–5.5 μm (x̄ = 20.5 × 4.3 μm, n = 20), uni to bi-seriate, partially overlapping, hyaline, fusiform, 1(–3)-septate, constricted at the central septum, filled with a different sized guttule per cell and surrounded by a mucilaginous sheath. Asexual morph: hyphomycetous. Colonies on PDA moderately growing, circular with a wavy margin, cottony on the surface, grayish brown, with reverse dark brown. Conidiophores 2.5–4 μm wide, micronematous to semi-macronematous, arising singly and not in sporodochia or synemmata, pale brown, unbranched, similar to the mycelial hypha. Conidiogenous cells 4.2–9.8 × 2.3–3.4 μm (x̄ = 6.9 × 2.9μm, n = 10), integrated, terminal, holoblastic, short-cylindrical to elongate-cylindrical, brown to pale brown smooth-walled. Conidia 6.6–10 × 5.6–7.5 μm (x̄ = 8.3 × 6.5 μm, n = 10) solitary, dry, elongate-clavate, pale to dark brown, 1–3-septate, evenly pigmented, constricted at the septa, smooth-walled.
Culture characteristics: Ascospores germinating on PDA within 18 h and producing germ tubes from both ends. Colonies on PDA attaining 40 mm diam. after 5 weeks at 25 °C, flat, grayish-brown, with reverse dark brown, with a crenate edge. After 2 weeks the culture produces lightly pigmented chemical exudate. Mycelium well-developed, superficial, composed of pale brown, thin-walled hyphae, frequently septate, smooth-walled. Chlamydospores sometimes formed individually, pale brown, broadly globose to subglobose.
Material examined: ITALY, Province of Forlì-Cesena [FC], Strada San Zeno, Galeata, on dead branches of Populus sp. (Salicaceae), 15 November 2012, Erio Camporesi IT 896 (MFLU 14-0588, holotype); ibid., (HHUF 30460, isotype); ex-type living culture, MFLUCC 13-0034, ICMP 20742.
Figure x. Angustimassarina populi (holotype). a–b Ascomata immersed on host surface c Section through ascomata (immersed in host fungi) d Peridium e Pseudoparaphyses f–h Mature bitunicate asci i–k Ascospores l Germinating ascospore. Scale bars: c = 100 μm, d–h = 20 μm, i– l = 10 μm.
Figure x. Angustimassarina populi (Asexual morph from the ex-type living culture). m–n Culture on PDA o–r Conidiogenesis and developing conidia. Scale bars: o–s = 10 μm.
References:
Thambugala KM, Hyde KD, Tanaka K, Tian Q et al. 2015 – Towards a natural classification and backbone tree for Lophiostomataceae, Floricolaceae, and Amorosiaceae fam. nov. Fungal Diversity 74, 199–266.
Last Update: 11 June 2021
Angustimassarina populi Thambug. & K.D. Hyde, in Thambugala et al., Fungal Diversity: 10.1007/s13225-015-0348-3, [56] (2015)
Index Fungorum number: IF 551279; Facesofungi number: FoF 01086
Saprotrophic on dead and aerial branches of Vitis vinifera. Sexual morph: Ascomata 150– 170 μm high, 110–120 μm (x̄ =160 × 138 μm, n = 10) diam., solitary, immersed, becoming erumpent, coriaceous, black, globose to subglobose, uniloculate, ostiolate. Ostiole crest-like, rounded, papillate, with a pore-like opening. Peridium 13–30 μm (x̄ = 21 μm, n = 10) wide, composed of dark brown to hyaline cells of textura angularis. Hamathecium comprising 1–2 μm wide, septate, unbranched, cellular pseudoparaphyses, embedded in a gelatinous matrix. Asci 82–95 × 9–14 μm (x̄ = 88 × 12 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical, pedicellate, rounded at the apex, with an ocular chamber. Ascospores 18–24 × 3–6 μm (x̄ = 21 × 4 μm, n = 20), uni- to bi-seriate, partially overlapping, hyaline, fusiform, 1-septate, constricted at the septum, filled with a different sized guttule in each cell, surrounded by a mucilaginous sheath. Asexual morph: Not observed.
Culture characteristics: Colonies on PDA attaining 45 mm diam. Mycelium well developed, superficial. Chlamydospores not observed. At 25° C, after 5 weeks, flat, greyish brown, with reverse dark greyish, with crenate edge.
Material examined: ITALY, Province of Forlì-Cesena, Teodorano - Meldola, on dead and aerial branch of Vitis vinifera (Vitaceae), 15 February 2015, Erio Camporesi IT 2378 (MFLU 15-1083); culture, MFLUCC 17-1217; ITALY, Province of Forlì-Cesena, Teodorano - Meldola, on dead and aerial branch of Vitis vinifera (Vitaceae), 21 February 2015, Erio Camporesi IT 2383 (MFLU 15-3508); culture, MFLUCC 17-1069.
Notes: This species is morphologically similar to A. populi and in the phylogenetic analysis of combined ITS, LSU, SSU and TEF1 sequence data, the isolates cluster with A. populi (Jayawardena et al. 2018). This study provides the first report of A. populi from Vitis species.
Figure x. Angustimassarina populi. a. Appearance of immersed ascomata on host surface. b. Section through ascoma. c. Immature asci. d. Mature bitunicate ascus. e. Ascospores. f, g. Culture on PDA. Scale bars: b=100 μm, c–d=20 μm, e=10 μm.
References:
Jayawardena RS, Hyde KD, Chethana KW, Daranagama DA et al. 2018 – Saprotrophic fungi on Vitis in China, Italy, Russia and Thailand. Mycosphere notes 9, 1–114.
Last Update: 16 September 2021