Pleosporales » Didymellaceae » Ascochyta

Ascochyta premilcurensis

Ascochyta premilcurensis (Tibpromma, Camporesi & K.D. Hyde) Qian Chen, Crous & L. Cai, in Chen, Hou, Crous & Cai, Stud. Mycol. 87: 128 (2017)

Synonymy: Heracleicola premilcurensis Tibpromma, Camporesi & K.D. Hyde, in Ariyawansa et al., Fungal Diversity: 10.1007/s13225-015-0346-5, [33] (2015)

Index Fungorum number: IF 820001; Facesofungi number: FoF 00921

Etymology: refers to the name of the municipality in Italy where the species was collected

Holotype: MFLU 15-1311.

Description: taken from Ariyawansa et al. (2015)

Saprobic on decaying plant stem of Heracleum sphondylium. Sexual morph: Ascomata 211–272 μm high × 150–221 μm diam. (x̄ = 254 × 196 μm, n = 5), immersed, visible as shiny, raised dots on the host surface, vaselike, solitary or scattered, with central short papilla, dark brown to black. Peridium 37–43 μm wide, a single stratum, comprising relatively large (6–9 μm), thick-walled, dark brown cells of textura angularis to textura globulosa. Hamathecium comprising numerous, 0.7–1.3 μm wide, long, filiform, frequently anastomosing, cellular, pseudoparaphyses. Asci 50–94 × 11–16 μm (x̄ = 72 × 14 μm, n = 10), 8-spored, bitunicate, fissitunicate, cylindric-clavate, short pedicellate, rounded at the apex, with a wide, shallow, ocular chamber. Ascospores 23–36 × 6–9 μm (x̄ = 27 × 7 μm, n = 15), overlapping 1–2-seriate, hyaline, 3-septate, fusoid with rounded ends, cell above central septum often enlarged, constricted at the septum, guttulate, smooth-walled, lacking a mucilaginous sheath. Asexual morph: undetermined.

Culture characteristics: on MEA reaching 4 cm diam. after 1 week at 16 °C, later with dense mycelium, with irregular, rough margin, flattened, brown to black; hyphae septate, branched, light-brown, thick-walled.

Material examined: ITALY, Premilcuore, Province of Forlì-Cesena, Valbura, on dead stem of Heracleum sphondylium (Apiaceae), 6 June 2014, Erio Camporesi IT1916 (MFLU 14-0725, holotype, HKAS, isotype); extype living culture, MFLUCC 14-0518, KUN; Ibid. (MFLU 15-1475, HKAS, isotypes); (MFLU 15-1476 bis, MFLU 15-1477 tris, paratypes).

GenBank Numbers: ITS: KT326694, LSU: KT326695.

Notes: The genus Heracleicola was introduced by Ariyawansa et al. (2015) to accommodate a single species Heracleicola premilcurensis, which is located in the genus Ascochyta based on combined LSU and ITS analysis in Chen et al. (2017). Heracleicola is therefore synonymized under Ascochyta, and a new combination in Ascochyta was proposed (Chen et al. 2017).