Didysimulans mezzanensis
Didysimulans mezzanensis Tibpromma, Camporesi & K.D. Hyde, in Tibpromma et al., Fungal Diversity 83: 79 (2017)
Index Fungorum number: IF 552772; Facesofungi number: FoF 2886
Etymology: Named after the province which the fungus was found.
Holotype: MFLU 15-1490
Saprobic on dead stem of Thalictrum sp. Sexual morph: Ascomata 83–95 μm high × 110–139 μm diam. (x̄ = 89 × 124 μm, n = 5), immersed in host tissues, solitary, black, irregular, conspicuous at the surface, ostiolate. Peridium 6–29 μm wide, composed of two strata, an outer stratum of thin-walled and brown cells arranged in a textura angularis and inner layer of hyaline cells of textura angularis. Hamathecium comprising 0.8–1.2 μm wide, dense, septate, guttulate pseudoparaphyses. Asci 45–60 × 10–13 μm (x̄ = 53 × 12 μm, n = 15), 8-spored, bitunicate, cylindrical, slightly curved, short pedicellate, apically rounded. Ascospores 13–16 × 4–8 μm (x̄ = 15 × 7 μm, n = 20), overlapping 1–2-seriate, 2-celled, hyaline, 1-septate, guttulate, enlarged at the first cell, constricted at the septum, conical at the ends, smooth-walled, lacking a mucilaginous sheath. Asexual morph: Colonies superficial to immersed;
mycelium composed of septate, branched, thick-walled, smooth, guttulate, yellow green, coiled, smooth, hyphae, producing thick-walled, small chlamydospores; sometimes, hyphae packed into knot-like structures.
Culture characteristics: Ascospore germinating on MEA within 12 h. Colonies on MEA reaching 5 mm diam.in 7 days at 16 °C, rhizoidal with lobate edge, rough, flat on surface, hyaline at beginning and becoming yellow green, with septate, branched, guttulate, hyphae packed in circular knots; not sporulating in culture.
Material examined: ITALY, near Mezzana Province, Trento, dead stem of Thalictrum sp. (Ranunculaceae), 16 July 2014, Erio Camporesi, IT1998A (MFLU 15-1490, holotype); ex-type living culture, MFLUCC 15-0067); Ibid. (HKAS 94598 bis, paratype).
GenBank Numbers: LSU:KY496733, ITS:KY496753, SSU:KY501121, RPB2:KY514411.
Notes: Didysimulans mezzanensis is introduced as the type species of Didysimulans. ITS nucleotide sequence comparison reveals eight base pair differences between D. mezzanensis and D. italica (Tibpromma et al. 2017).
Figure x. Didysimulans mezzanensis (MFLUCC 15-0067, ex-type living culture on WA). a Growth of asexual morph on plant tissues. b Growth of asexual morph on WA. c, e Squash mount of vegetative hyphae. Scale bars a, b = 200 μm, c = 20 μm, d, e = 10 μm.
References:
Tibpromma S, Hyde KD, Jeewon R, Maharachchikumbura SS et al. 2017 – Fungal diversity notes 491–602: taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity 83, 1-261.
Last update: 01 July 2021