Pleosporales » Didymellaceae » Didysimulans

Didysimulans mezzanensis

Didysimulans mezzanensis Tibpromma, Camporesi & K.D. Hyde, in Tibpromma et al., Fungal Diversity 83: 79 (2017)

Index Fungorum number: IF 552772; Facesofungi number: FoF 2886

Etymology: Named after the province which the fungus was found.

Holotype: MFLU 15-1490

Saprobic on dead stem of Thalictrum sp. Sexual morph: Ascomata 83–95 μm high × 110–139 μm diam. (x̄ = 89 × 124 μm, n = 5), immersed in host tissues, solitary, black, irregular, conspicuous at the surface, ostiolate. Peridium 6–29 μm wide, composed of two strata, an outer stratum of thin-walled and brown cells arranged in a textura angularis and inner layer of hyaline cells of textura angularis. Hamathecium comprising 0.8–1.2 μm wide, dense, septate, guttulate pseudoparaphyses. Asci 45–60 × 10–13 μm (x̄ = 53 × 12 μm, n = 15), 8-spored, bitunicate, cylindrical, slightly curved, short pedicellate, apically rounded. Ascospores 13–16 × 4–8 μm (x̄ = 15 × 7 μm, n = 20), overlapping 1–2-seriate, 2-celled, hyaline, 1-septate, guttulate, enlarged at the first cell, constricted at the septum, conical at the ends, smooth-walled, lacking a mucilaginous sheath. Asexual morph: Colonies superficial to immersed;

mycelium composed of septate, branched, thick-walled, smooth, guttulate, yellow green, coiled, smooth, hyphae, producing thick-walled, small chlamydospores; sometimes, hyphae packed into knot-like structures.

            Culture characteristics: Ascospore germinating on MEA within 12 h. Colonies on MEA reaching 5 mm diam.in 7 days at 16 °C, rhizoidal with lobate edge, rough, flat on surface, hyaline at beginning and becoming yellow green, with septate, branched, guttulate, hyphae packed in circular knots; not sporulating in culture.

Material examined: ITALY, near Mezzana Province, Trento, dead stem of Thalictrum sp. (Ranunculaceae), 16 July 2014, Erio Camporesi, IT1998A (MFLU 15-1490, holotype); ex-type living culture, MFLUCC 15-0067); Ibid. (HKAS 94598 bis, paratype).

GenBank Numbers: LSU:KY496733, ITS:KY496753, SSU:KY501121, RPB2:KY514411.

Notes: Didysimulans mezzanensis is introduced as the type species of Didysimulans. ITS nucleotide sequence comparison reveals eight base pair differences between D. mezzanensis and D. italica (Tibpromma et al. 2017).