Pleosporales » Didymosphaeriaceae » Kalmusia

Kalmusia spartii

Kalmusia spartii Wanas., Camporesi, E.B.G. Jones & K.D. Hyde, in Liu et al., Fungal Diversity: 10.1007/s13225-015-0324-y, [87] (2015)

Index Fungorum number: IF 550895; Facesofungi number: FoF 00385

Etymology: Named after the host genus from which it was collected, Spartium

Holotype: MFLU 14-0751.

Saprobic on dead herbaceous branches. Sexual morph: Ascomata 350–450 μm high × 250–400 μm diam. (x̄ = 395.3 × 323.7 μm, n = 10), solitary, scattered, immersed to erumpent, globose or subglobose, coriaceous, wall black, with or without papilla, ostiolate. Ostiole 150–200 μm high, 90–130 μm diam. (x̄ =174.3 × 109 μm, n = 10), blackish-brown, smooth, ostiolar canal filled with hyaline cells. Peridium 15–30 μm wide at the base, 25–50 μm wide at the sides, thick, 1-layered, composed of small heavily pigmented thick-walled cells of textura angularis. Hamathecium comprising numerous, 1.9 μm (n = 30) wide, filamentous, branched septate, pseudoparaphyses. Asci 110–130 × 10–16 μm (x̄ = 119.6 × 13.9 μm, n = 40), 8-spored, bitunicate, fissitunicate, clavate, with a long, narrow, furcate pedicel, up to 30–35 μm long, with a minute ocular chamber. Ascospores 19–22 × 5.5–9 μm (x̄ = 20.2 × 7.6 μm, n = 50), overlapping 1–2-seriate, narrowly ovoid to clavate, 3-distoseptate, constricted at the septa, initially hyaline, becoming yellowish-brown at maturity, narrowly rounded at both ends, smooth-walled, not surrounded by a mucilaginous sheath. Asexual morph: Conidiomata 300–550 μm diam., 200–250 μm high, superficial or immersed in the agar, dark brown to black, clothed with white hyphal projections, simple cavities. Conidiomatal wall composed of a 40–50 μm wide outer layer and 10–20 μm wide inner layer of cells of textura angularis. Conidiogenous cells 5–8 × 2–4 μm discrete, assembled into protruding masses or integrated in very compact conidiophores. Conidia 2.5–4 × 1.5–2.5 μm (x̄ = 3.2 × 1.9 μm, n = 20) narrowly ellipsoidal or short-cylindrical, straight or slightly curved, rounded at both ends, one-celled, with one or two small, polar guttules, initially hyaline, becoming light brown, smooth-walled.

Culture characters: Colonies on PDA reaching a diam. of 30–35 mm in 21 d, flat, with undulate to lobate margin, hyaline, covered by thin, felty, white, aerial mycelium, honey-yellow in reverse, sporulation after 8 weeks.

Material examined: ITALY, Forlì-Cesena Province: Castello di Corniolo, Santa Sofia, dead and hanging branches of Spartium junceum L. (Fabaceae), 15 March 2013, E. Camporesi (MFLU 14–0751, holotype); ex-type living culture, MFLUCC 14–0560.

GenBank ITS: KP744441, LSU: KP744487, SSU: KP753953.

Notes: Multi-gene phylogenetic analyses indicated that Kalmusia spartii belongs to Didymosphaeriaceae and grouped together with other Kalmusia strains. Kalmusia spartii has a similar morphology to K. ebuli, but in our phylogenetic tree, K. ebuli and K. spartii were well resolved.