Pleosporales » Didymosphaeriaceae » Montagnula

Montagnula cirsii

Montagnula cirsii Qing Tian, Camporesi & K.D. Hyde, in Hyde et al., Fungal Diversity 80: 43 (2016)

Index Fungorum number: IF 552184; Facesofungi number: FoF 02255

Etymology: In reference to the host genus Cirsium, from which this holotype was collected.

Holotype: MFLU 14-0730.

Saprobic under periderm or semi-immersed in woody plant substrates. Sexual morph: Ascomata 385–415 μm diam. × 510–525 μm high (x̄ = 400 × 515.5 μm, n = 10), semi-immersed to erumpent, solitary, scattered, or sometimes gregarious, globose, black, smooth-walled, neck long, with a small, flattened, ellipsoid ostiole at the apex. Peridium 41–58.5 μm (x̄ = 43.3 μm, n = 6), 2-layered, the outer layer composed of irregular, thick-walled, brown cells of textura angularis; the inner layer composed of hyaline, smaller cells of textura angularis. Hamathecium comprising 1–2 μm broad, septate, long, colourless, branched or simple pseudoparaphyses, surrounding the asci. Asci 84.5–119.5 × 10.5–13.5 μm (x̄ = 101 × 12 μm, n = 10), 8-spored, bitunicate, clavate, long pedicellate, apically rounded with an ocular chamber. Ascospores 18– 23.5 × 6.5–9.5 μm (x̄ = 21.5 × 8 μm, n = 10), overlapping uni-seriate or uni-seriate, 3-septate, ellipsoid to fusiform, curved, yellow to brown, constricted at the septa, broader at the middle two cells, tapering or obtuse at both ends, smooth-walled. Asexual morph: Undetermined.

Culture characteristics:  Ascospore germinating on PDA within 12 h. Colonies on PDA, reaching 8 mm diam. In 7 days at 25 °C. Mycelium superficial, hyaline, hairy, with entire edge, floccose at the center, drift white from above and light brown at the center from below.

Material examined: ITALY, Province of Forlı`-Cesena [FC], Balze-Verghereto, on dead stem of Cirsium sp. (Compositae), 21 May 2013, Erio Camporesi (MFLU 14-0730, holotype); ibid., (HKAS 94523, isotype); ex-type living culture, MFLUCC 13-0680, KUMCC 16-0018.

GenBank:

Notes: Montagnula cirsii is morphologically similar to M. bellevaliae and M. scabiosae (Hongsanan et al. 2015). However, they can be distinguished by various characters, such as host, shape of ascomata and different sizes of asci. Montagnula bellevaliae occurs on dead stems of Bellevalia romana, M. scabiosae on dead stems of Scabiosa sp. and M. cirsii on dead stems of Cirsium sp. The orientation of ascomata is also different. Montagnula bellevaliae has an eccentric papilla, while the other two species have regular papilla. The size of asci is also quite different; Montagnula cirsii is smaller than M. scabiosae (84.5–119.5 × 10.5–13.5 μm vs. 110–130 × 14–20 μm) but larger than M. bellevaliae which is 70–100 μm in length, 9–12 μm in width.). Based on phylogenetic analysis of Hyde et al. (2016), M. cirsii clusters with M. scabiosae in Montagnula with relatively high support (99 % MP /1.00 PP). We therefore introduce a new species based both on morphology and phylogeny (Hyde et al. 2016).