Pseudocamarosporium pini
Pseudocamarosporium pini (Westend.) Phukhams., Camporesi & K.D. Hyde, in Li et al., Fungal Diversity 78: 10.1007/s13225-016-0366-9, [31] (2016)
Index Fungorum number: IF 551896; Facesofungi number: FoF 01817
Basionym: Hendersonia pini Westend., Bull. Acad. R. Sci. Belg., Cl. Sci.: tab. 9, no. 7 (1857)
≡ Camarosporium pini (Westend.) Sacc., Syll. fung. (Abellini) 3: 465 (1884)
Saprobic on dead cone of Pinus nigra J.F. Arnold. Sexual morph: Undetermined. Asexual morph: Conidiomata 105–174 μm high × 188–244 wide μm (x̄ = 145 × 210 μm, n = 5) diam., pycnidial, solitary, uniloculate, scattered, immersed to erumpent, subglobose, but sometimes irregular, brown to dark brown, ostiole central. Pycnidial wall 14–28 μm (–40 μm at apex), composed of 5 layers of brown-walled cells of textura angularis, hyaline inner layer lining bearing conidiogenous cells. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 2–6 × 3–5 μm (x̄ = 4 × 4 μm, n = 20) diam., enteroblastic, phialidic, determinate, smooth-walled, hyaline. Conidia 7–18 × 4–8 μm (x̄ = 14 × 6 μm, n = 50), oval to oblong, curved at the apex, with 1–3 transverse septa, and 1–2 longitudinal septa at the second and third cells, initially hyaline, brown to dark brown at maturity, narrowly rounded at both ends, smooth-walled.
Culture characteristics: Colonies on PDA 60 mm diam. after 4 weeks at 16 °C, cream to white at the margins, pale-brown to yellowish at the center; reverse yellowish to cream and orangish-white at the center, medium dense, circular, umbonate, fairly fluffy, without diffusible pigments.
Material examined: ITALY, Forlì-Cesena Province, Monte Mirabello - Predappio, on dead and terrestrial cone of Pinus nigra (Pinaceae), 13 October 2014, E. Camporesi (MFLU 15–3290, HKAS 91937, reference specimen designed here), ex-type living culture, MFLUCC 14–1091, KUMCC 15–0550.
Notes: Several Camarosporium species has been reported from Pinus spp., such as C. propinquum (Sacc.) Sacc., C. brabeji Marincowitz et al., and C. pini (Westend.) Sacc. (Grove 1937, Botella et al. 2010, Botella and Diez 2011). Wijayawardene et al. (2014) treated C. propinquum under Pseudocamarosporium typified by P. propinquum. The strain clustered in Didymosphaeriaceae, separate from the type of Camarosporium, C. quaternatum, which clustered in Pleosporinae. Crous et al. (2015) synonymized Camarosporium brabeji Marincowitz et al. under Pseudocamarosporium brabeji as the molecular data placed them in Didymosphaeriaceae. Camarosporium pini was originally described by Westendorp (1857) as Hendersonia pini, and the species is recorded from Pinus silvestris (Grove 1937). When comparing the morphology of our species with C. pini, they are similar in the host and morphology. The conidiomata are similar in size, with thick walls up to 40 μm wide. The dimension of conidia overlap and are oblong, rounded at both ends, with one or two longitudinal septa in the middle cells. Based on phylogenetic analysis in this study, our strain clusters within Pseudocamarosporium in Didymosphaeriaceae with relatively high support (92 % MP/88 % ML /0.99 PP). We, therefore, synonymize Camarosporium pini under Pseudocamarosporium pini based on morphology and phylogeny and designate our collection as a reference specimen (sensu Ariyawansa et al. 2014), which we illustrate here (Li et al. 2016).
Figure x. Pseudocamarosporium pini (MFLU 15–3290, reference specimen) a, b Appearance of conidiomata on Pinus nigra cone c Vertical section of conidioma d Peridium e Ostiole f–I Developing stages of conidia j–o Conidia p–q Culture characters on PDA. Scale bars: c = 100 μm, d–e = 20 μm, f–o = 5 μm, p– q = 30 mm.
References:
Ariyawansa HA, Hawksworth DL, Hyde KD, Jones EBG et al. 2014 – Epitypification and neotypification: guidelines with appropriate and inappropriate examples. Fungal Diversity 69, 57–91.
Botella L, Santamaría O, Diez JJ 2010 – Fungi associated with the decline of Pinus halepensis in Spain. Fungal Diversity 40, 1–11.
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Grove WB 1937 – British stem-and leaf-fungi (Coelomycetes). Vol. II.Cambridge at the University Press, Cambridge.
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Westendorp GD 1857 – Sur quelques hypoxylées inédites ou nouvelles pour la flore de la Belgique. Bull de l’Académie Royale de Belgique Classe de Sci, Sér 22, 554–579.
Wijayawardene NN, Hyde KD, Bhat DJ, Camporesi E et al. 2014 – Camarosporium-like species are polyphyletic in Pleosporales; introducing Paracamarosporium and Pseudocamarosporium gen. nov. in Montagnulaceae. Cryptogamie Mycologie 35, 177–198.
Last Update: 15 December 2021