Fuscostagonospora camporesii
Fuscostagonospora camporesii D.S Tennakoon, K.D Hyde, in Hyde et al., Fungal Diversity 100: 60 (2020)
Index Fungorum number: IF557123; Facesofungi number: FoF 06333
Etymology: In honour of Mr. Erio Camporesi for his immense contribution to mycology
Holotype: MFLU 16-1362
Saprobic on dead branches of Cornus sanguinea. Sexual morph: Ascomata 100–130 μm high, 160–220 μm diam., solitary, scattered to clustered, immersed, visible as raised, black spots on host surface globose to subglobose, glabrous, uni-loculate, ostiole central with minute papilla. Peridium 22–33 μm wide, thin to thick-walled with equal thickness, composed of two type layers of pseudoparenchymatous cells, inner layer comprising 3–5 cell layers of flattened, hyaline cells, arranged in a textura prismatica, outer layer comprising several layers of dark brown to black cells, arranged in a textura angularis. Hamathecium composed of dense, broad, 1.8–2.5 μm wide, filamentous, cellular pseudoparaphyses, with indistinct septa, not constricted at the septa, anastomosing at the apex, embedded in a hyaline gelatinous matrix. Asci (69–)80–90(–110) × (7–)8–9(–9.5) μm (x̄ = 86 × 8.5 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindric-clavate, short pedicellate, with furcate to obtuse end, apically rounded with well-developed ocular chamber. Ascospores (11.8–)13–15(–15.7) × (5.5–)6–6.5(–7.3) μm (x̄ = 13.9 × 6.3 μm, n = 40), overlapping, 1-seriate, ellipsoid to obovoid, initially hyaline to slightly brown, becoming brown at maturity, aseptate when young, becoming 1-septate, straight to slightly curved, smooth-walled. Asexual morph: Undetermined.
Culture characteristics: Colonies on PDA reaching 35–40 mm diam. after 5 weeks at 20–25 °C, colonies medium sparse, circular, convex, slightly rough surface with edge entire, well defined margin, cottony to fairly fluffy with sparse aspects, colony from above, white to cream at the margin, white to yellowish at the centre; from below, yellow to yellowish brown at the margin, light brown at the centre, mycelium white to cream with tufting, not producing pigmentation in PDA media agar.
Material examined: ITALY, Province of Forlì-Cesena [FC], Monte Maggiore-Predappio, on dead branches of Cornus sanguinea L. (Cornaceae), 9 March 2015, E. Camporesi, IT 2409 (MFLU 16-1362, holotype); ibid., KUNHKAS 9370 (isotype), ex-type living culture, MFLUCC 16-0787, KUMCC 15-0554.
GenBank Numbers: ITS: MN750611; LSU: MN750590; SSU: MN750605.
Note: The morphological characteristics of Fuscostagonospora camporesii fit with F. cytisi Jayasiri, Camporesi & K.D. Hyde (MFLU 15-3607) in having immersed, subglobose to globose ascomata, cylindric-clavate, short pedicellate asci and 1-septate, ellipsoid to obovoid ascospores and trabeculate pseudoparaphyses (Hyde et al. 2017). But, F. camporesii differs from F. cytisi in having brown and smaller ascospores (13.9 × 6.3 μm versus 16 × 7.4 μm), whereas, F. cytisi has hyaline ascospores with a mucilaginous sheath (Hyde et al. 2017). We also compared the sequences of our newly-described taxon with F. cytisi. According to the multi-gene phylogenetic analyses of a combined LSU, SSU, ITS and TEF1-α sequence dataset, Fuscostagonospora camporesii is closely related to other Fuscostagonospora species, in particularly with F. banksiae (CBS 144621) with significant support (89% ML, 79% MP). The asexual morph of F. banksiae was isolated from Banksia sp. (Proteaceae) and introduced by Crous et al. (2019). A comparison of the 665 nucleotides across the ITS (+5.8S) gene region of F. camporesii and F. banksiae shows 30 base pair differences (4.51%) which justifies the new species following the guidelines of Jeewon and Hyde (2016).
Figure x. Fuscostagonospora camporesii (MFLU 16–1362, holotype). a Ascomata visible as black dots on host surface. b–c Vertical sections of ascomata. d Section through peridium. e Pseudoparaphyses. f–i Asci. j–n Ascospores. o Germinated ascospore. p Colony from above. q Colony from below. Scale bars: b–c = 50 μm, d–i = 20 μm, j–n = 5 μm, o = 10 μm.
References:
Crous PW, Schumacher RK, Akulov A, Thangavel R et al. 2019 – New and interesting fungi 2. Fungal Systematics and Evolution 3, 57–134.
Hyde KD, Norphanphoun C, Abreu VP, Bazzicalupo A et al. 2017 – Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. Fungal Diversity 87, 1–235.
Hyde KD, Dong Y, Phookamsak R, Jeewon R et al. 2020 – Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 16, 1–273.
Jeewon R, Hyde KD 2016 – Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7, 1669–1677.