Keissleriella camporesiana
Keissleriella camporesiana Phukhams., Camporesi. & K.D. Hyde, in Hyde et al., Fungal Diversity 100: 63 (2020)
Index Fungorum number: IF556759; Facesofungi number: FoF 06344
Etymology: The species epithet was honouring Erio Camporesi.
Holotype: MFLU 15-3288
Saprobic on dead stems of Centaurea L. Sexual morph: Ascomata 170–240 × 270–320 μm (x̄ = 165 × 240 μm, n = 5), on surface of the host, covered by a pseudoclypeus, immersed to superficial, solitary, scattered, uni-loculate, obpyriform to compressed globose, flatten base, brown to dark brown, rough-walled, papillate. Ostiole central, 55 × 49 μm, brown to dark brown, papillate, opened pore, easily open, ostiolate with black setae on the papilla. Peridium 16–40 μm wide, thick, multi-layer, comprising of 7–9 layers of dark brown to brown cells of textura angularis, inner layers comprising thin, hyaline cells. Hamathecium of dense, 1.15–2.3 μm wide (x̄ = 1.5 μm, n = 50), filiform branches, anastomosing above asci, reaching the ostiole part, transverse septate, trabeculate pseudoparaphyses. Asci 68–120 × 15–20 μm (x̄ = 95 × 20 μm, n = 40), 8-spored, bitunicate, fissitunicate, thick-walled, cylindric-clavate to clavate, apically rounded, with furcated pedicel, ocular chamber clearly visible when immature. Ascospores 20–30 × 7–8 μm (x̄ = 26 × 7.5 μm, n = 50), bi-seriate, partial overlapping, hyaline, broad fusiform, cell above median septum slightly wider than below, lower part longer than the upper part, tapering towards the ends, slightly acute at both ends, with (1–)4–5 transverse septa, constricted at the septa, large guttules each cell, deeply constricted at the median septum, smooth-walled, with mucilaginous sheath. Asexual morph: Undetermined.
Culture characteristics: Colonies on MEA, reaching 30 mm diam. after 4 weeks of incubation at 16 °C. Culture grey brown, with orange brown mixed in the mycelium after 4 weeks, dense mycelia, colonies circular, flat, umbonate, dull, covered with aerial mycelium, white mycelium at the edges; reverse dark brown, dense, irregular, fimbriate margin.
Material examined: ITALY, Province of Forlì-Cesena [FC], Strada San Zeno-Galeata, on dead stems of Centaurea L. (Asteraceae), 22 December 2012, E. Camporesi, IT 971 (MFLU 15-3288, holotype), ex-type living culture, MFLUCC 15-0029.
GenBank Numbers: ITS: MN401745; LSU: MN40174; SSU: MN401743: TEF1-α; MN397907.
Note: Keissleriella is characterized by ascomata with black setae on the papilla (Zhang et al. 2012) and trabeculate pseudoparaphyses (Liew et al. 2000). In the phylogenetic analyses of this study, K. camporesiana (strain MFLUCC 15-0029) and K. dactylis Singtr., Camporesi & K.D. Hyde (strain MFLUCC 13-0751) form a separate subclade with moderate support (75% ML, 0.94 BYPP). Keissleriella camporesiana is morphologically distinguished from K. dactylis by the second cell of ascospores being enlarged and being 4–5-septate (Singtripop et al. 2015). Keissleriella dactylis was described from the host family Poaceae while K. camporesiana occurs on Asteraceae.
Figure x. Keissleriella camporesiana (MFLU 15–3288, holotype). a Ascomata on Centaurea. b Close up of ascomata. c Vertical section of ascoma. d Ostiolar canal contain of brown setae. e Peridium. f Pseudoparaphyses. g–i Developing state of asci. j–n Developing state of ascospores. o Ascospore stained with India ink showed mucilaginous sheath. Scale bar: b = 500 μm, c = 100 μm, d = 20 μm, e–i = 50 μm, j–o = 10 μm.
References:
Hyde KD, Dong Y, Phookamsak R, Jeewon R et al. 2020 – Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 16, 1–273.
Liew ECY, Aptroot A, Hyde KD 2000 – Phylogenetic significance of the pseudoparaphyses in Loculoascomycete taxonomy. Molecular Phylogenetics and Evolution 16, 392–402.
Singtripop C, Camporesi E, Ariyawansa HA, Wanasinghe DN et al. 2015 – Keissleriella dactylidis, sp. nov., from Dactylis glomerata and its phylogenetic placement. Science Asia 41, 295–304.
Zhang Y, Crous PW, Schoch CL, Hyde KD 2012 – Pleosporales. Fungal Diversity 53, 1–221.