Murilentithecium rosae
Murilentithecium rosae Phukhams., Camporesi & K.D. Hyde, in Wanasinghe et al., Fungal Diversity: 10.1007/s13225-018-0395-7, [51] (2018)
Index Fungorum number: IF554139; Facesofungi number: FoF 03978
Etymology: specific epithet reflects the host genus Rosa.
Holotype: MFLU 15-1043.
Saprobic on dead spines of Rosa. Sexual morph: Undetermined. Asexual morph: Conidiomata pycnidial, 97–201 μm high × 181–270 μm diam (x̄ = 135 × 225 μm, n = 10), solitary or gregarious, black, immersed, sometimes scattered beneath the host tissues, partly erumpent, unilocular, with apapillate ostioles. Ostiole central, filled with hyaline or brown cells. Pycnidial wall multi-layered, 9–30 μm wide at the sides, up to 35 μm wide at the apex comprising 2 layers, outer layer heavily pigmented, thick walled, comprising blackish or to dark reddish-brown cells of textura angularis, cells towards the inside lighter, inner layer composed of hyaline, thin-walled cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 4–12 × 3–9 μm (x̄ = 7 × 6 μm; n = 30), blastic, phialidic, discrete, determinate, hyaline, smooth. Conidia 15–17 × 7–9 μm (x̄ = 16 × 8 μm, n = 40), oval, straight to slightly curved, rounded at both ends, with 3 transverse septa, and 1–2 longitudinal septa, muriform, smooth-walled, yellowish-brown to dark brown.
Material examined: ITALY, Province of Forlı`-Cesena, near Monte Fumaiolo, on dried aerial spines of Rosa canina L. (Rosaceae), 23 October 2014, Erio Camporesi, IT 2192 (MFLU 15-1043, holotype), ex-type living culture, MFLUCC 15-0044.
GenBank Numbers: ITS: MG828920; LSU: MG829030; SSU: MG829137.
Notes: Murilentithecium rosae shares some similar morphological characters with the asexual morph of M. clematidis in having pycnidial, immersed, uniloculate conidiomata, blastic, phialidic, hyaline conidiogenous cells and muriform conidia. Murilentithecium rosae can be distinguished from M. clematidis in having conidia with 3 transverse septa, and 1–2 longitudinal septa (15–17 × 7–9 μm), whereas M. clematidis has conidia with 3-5 transverse septa, and 2–5 longitudinal septa (13–18 9 5–8 lm). Based on the multi-gene phylogenetic analyses, Murilentithecium rosae is basal to M. clematidis with strong bootstrap support (78% ML, 1.00 BYPP).
Figure x. Murilentithecium rosae (MFLU 15-1043, holotype) a, b On spines of Rosa canina. c Close up of conidioma erumpent on host surface. d Vertical section of conidioma. e Ostiole. f Section of partial peridium layer. g–j Developing stages of conidia. k–p Developing stages of conidia. Scale bars: b–d = 200 μm, e–f = 50 μm, g–j = 10 μm, k–p = 10 μm
References:
Wanasinghe DN, Phukhamsakda C, Hyde KD, Jeewon R et al. 2018 – Fungal diversity notes 709–839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal diversity 89, 1–236.