Pleosporales » Lentitheciaceae » Pleurophoma

Pleurophoma italica

Pleurophoma italica Tibpromma, Camporesi & K.D. Hyde, in Tibpromma et al., Fungal Diversity 83: 62 (2017)

Index Fungorum number: IF 552689; Facesofungi number: FoF 2768

Etymology: refers to the country where the holotype was collected.

Holotype: MFLU 15-1254

Saprobic on cone of Pinus nigra J.F. Arnold lying on the ground. Sexual morph: Ascomata 154–167 μm high × 141–155 μm diam. (x̄ = 157 × 148 μm, n = 5), semi-immersed or erumpent, solitary, or in small groups, uniloculate, globose to subglobose, conspicuous at the surface, black, without hairs, ostiolate. Peridium 6–12 μm wide, comprising 4–5 layers of reddish-brown cells of textura angularis. Hamathecium 2.2–3.4 μm wide, comprising dense, aseptate, unbranched pseudoparaphyses. Asci 51–106 × 7–11 μm (x̄ = 72 × 9 μm, n = 15), 8-spored, bitunicate, fissitunicate, cylindrical, with short, club-shaped pedicel. Ascospores 13–16 × 3–5 μm (x̄ = 14 × 4 μm, n = 20), overlapping 1–2-seriate, hyaline, fusiform,1-septate in the center, deeply constricted at the septum, swollen at the first cell, conical at the ends, with guttulate, lacking a mucilaginous sheath. Asexual morph: Coelomycetous, produced on sterilized bamboo pieces and pine needles on water agar. Conidiomata 68–105 μm high × 77–98 μm diam., pycnidial, superficial on substrate, solitary, black, dull, uniloculate, globose to subglobose, conspicuous at the surface. Conidiophores reduced to conidiogenous cells arising from the innermost wall-layercells of the conidiomata. Conidiogenous cells 5.3–8.6 × 3.2–5.1 μm (x̄ = 6.6 × 3.9 μm, n = 20), holoblastic, phialidic, globose to oblong, hyaline, and formed from the inner layer of the pycnidium wall. Conidia 0.9–3 9 0.7–1.8 μm (x̄ = 1.9 9 1.2 μm, n = 40), globose to oval, aseptate, hyaline, smooth-walled, spermatia-like.

Culture characteristics: Colonies on MEA at 16 °C reaching 2.5 cm in 1 week, circular with wavy margin, white–grey, smooth surface and raised.

Material examined: ITALY, near Ortignano-Raggiolo, on cone of Pinus nigra (Pinaceae) on ground, 29 July 2014, Erio Camporesi, IT2024 (MFLU 15-1254, holotype); ex-type living culture MFLUCC 15-0061 (HKAS 94600 bis, paratype).

GenBank Numbers LSU:KY496734, ITS:KY496754, SSU:KY501122, TEF1:KY514398.

Notes: Pleurophoma italica is introduced as a new species with both sexual and asexual morphs. The phylogeny indicates a close relationship of P. italica to P. pleurospora (type species), but constitutes a separate branch in this study. Pleurophoma italica shares similar asexual morphology with P. pleurospora. However, our taxon also clustered with Keissleriella sparticola, K. cladophila and K. genistae, but the morphological characteristics of sexual morphs are different. Tibpromma et al. (2017) suggested that, if Keissleriella sparticola, K. cladophila and K. genistae asexual morphs are discovered with more phylogenetic evidence, these three species could be synonymized under Pleurophoma in the future. Hence, based on the phylogenetic analysis, Tibpromma et al. (2017) agree with Crous et al. (2015a), that the link between Pleurophoma and Keissleriella needs to be resolved.