Leptosphaeria ebuli Jayasiri, Camporesi & K.D. Hyde, in Liu et al., Fungal Diversity: 10.1007/s13225-015-0324-y,  (2015)
Index Fungorum number: IF 550958; Facesofungi number: FoF 00454
Etymology: In reference to its occurrence on Sambucus ebulus.
Holotype: MFLU 14-0820.
Saprobic on dead stem of Sambucus ebulus. Sexual morph: Ascomata 226–396 × 241–251 μm (x̄ =300 × 241μm, n = 10), solitary or scattered, initially immersed, becoming erumpent to near superficial, globose to subglobose, broadly or narrowly conical, coriaceous, smooth-walled, ostiolate. Ostiole usually broadly papillate, darkened at the base. Peridium 24–26 μm (x̄ = 45 μm, n = 20) wide, comprising two types of cells, outer cells of 1–2 layers of heavily pigmented cells of textura angularis, inner layer composed of small, light brown to hyaline cells of textura angularis. Hamathecium of septate, long, hyaline, cellular pseudoparaphyses, embedded in gelatinous matrix between and above the asci. Asci 80–109 × 8–9 μm (x̄ = 100 × 9 μm, n = 20), 8-spored, bitunicate, fissitunicate, clavate to sub-cylindrical, with a short, broad pedicel, thickened and rounded at apex with a distinct ocular chamber surrounded by a large, distinct, apical ring. Ascospores 23–28 × 4–5 μm (x̄ = 25 × 5.8 μm, n = 40), biseriate to overlapping tri-seriate, ellipsoidal with broadly rounded ends, hyaline to light brown when immature, becoming brown to chestnut brown when mature, 3-septate, smooth-walled, lacking a sheath. Asexual morph: Undetermined.
Culture characters: Ascospores germinated on MEA at 18 °C within 24 h and germ tubes were produced from both ends of ascospores. Colonies on MEA were growing fast (within one week 2 cm diam. colony), white-gray.
Material examined: ITALY, Province of Monte Fumaiolo (FC), on stems of Sambucus ebulus L. (Adoxaceae), 23 July 2014, E. Camporesi (MFLU 14-0820, holotype); ex-type living cultures, MFLUCC 14-0828.
GenBank ITS: KP744446, LSU: KP744488, SSU: KP753954.
Notes: Multi-gene phylogenetic analysis is also indicated that Leptosphaeria ebuli forms a distinct lineage sister to Leptosphaeria sydowii (BS support 96 %) which is known as an asexual morph (de Gruyter et al. 2012).The morphological characters of L. ebuli is fitted with the generic concept of Leptosphaeria in having superficial ascomata with a shiny papilla, multi-layered peridium comprising cells of textura angularis, cellular pseudoparaphyses and fissitunicate, narrowly cylindrical asci bearing multi septate, yellowish-brown to brown ascospores but differ from the generic type of L. doliolum in the shape of the ascomata (globose to subglobose versus conical), width of the ostiole (narrow versus broad) ostiole pore and the size of the ascospores (25× 5.8 μm versus 18 × 3 μm). Hence we introduced Leptosphaeria ebuli in Leptosphaeria as a new species based on both molecular data coupled with morphology.