Coelodictyosporium muriforme
Coelodictyosporium muriforme Thambug., Camporesi & K.D. Hyde, in Thambugala et al., Fungal Diversity 74: 218 (2015)
Index Fungorum number: IF 551237; Facesofungi number: FoF 00803
Etymology: Referring to its muriform ascospores.
Holotype: MFLU 14-0586
Saprobic on dead branches of Spartium junceum L. Sexual morph: Ascomata 400–700 μm high, 350–550 μm diam. (x̄ = 490 × 425 μm, n = 5), solitary or gregarious, immersed, coriaceous, black, globose to subglobose, ostiolate, apex well-developed. Ostiole crest-like, central, broadly papillate, with a pore-like opening. Peridium 25–60 μm wide (x̄ = 40 μm, n = 15), wider at the apex and thinner at the base, composed of two strata, outer stratum comprising dark brown to black, somewhat flattened cells of textura angularis, fusing and indistinguishable from the host tissues, and inner stratum comprising 2–3 layers of brown to lightly pigmented cells of textura angularis. Hamathecium comprising 1.5–3 μm wide, numerous, aseptate, unbranched, cellular pseudoparaphyses, anastomosing above the asci, embedded in a gelatinous matrix. Asci 85–135 × 12–17 μm (x̄ = 119 × 14.5 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindric-clavate, with a long pedicel, apically rounded, with an ocular chamber. Ascospores 17–23.5 × 6.5–9 μm (x̄ = 20 × 7.4 μm, n = 25), uni to bi-seriate, partially overlapping, yellowish to brown, ellipsoid to fusiform, muriform, 5–6 transversely septate, with 1–2 vertical septa, deeply constricted at the middle septum, upper part slightly wider, with small, pointed appendages at both ends and surrounded by a thick mucilaginous sheath. Asexual morph: Undetermined
Culture characteristics: Ascospores germinating on PDA within 18 h and germ tubes produced from several septa. Colonies on PDA reaching 10 mm diam. after 4 weeks at 25 °C, flat to effuse, surface white, reverse yellowish-orange, dense, aerial, surface smooth, with entire to slightly undulate edge.
Material examined: ITALY, Province of Forlì-Cesena [FC], Montebello, Modigliana, on dead branches of Spartium junceum (Fabaceae), 20 October 2012, Erio Camporesi IT 839 (MFLU 14-0586, holotype); ibid., (HHUF 30458, isotype); ex-type living culture, MFLUCC 13-0351, ICMP; ibid., Passo delle Forche, Galeata, on dead branches of Spartium junceum (Fabaceae), 28 February 2013, Erio Camporesi IT 839-2 (MFLU 15-1122, paratype); ibid., Marsignano, Predappio, on dead branches of Spartium junceum (Fabaceae), 14 March 2013, Erio Camporesi IT 839-3 (MFLU 15-1123, paratype).
GenBank:
Figure x. Coelodictyosporiu muriforme (holotype). a, b Appearance of ascomata on the host tissue c. Section through ascoma d. Ostiole (note the welldeveloped apex) e. Section of the peridium f. Pseudoparaphyses g Immature ascus h–i Mature bitunicate asci j–l. Ascospores (note the small appendages in k) m. Ascospore stained in Indian ink. Scale bars: c-d=100 μm, e, f=25 μm, g–i=50 μm, j–m=25 μm
References:
Thambugala KM, Hyde KD, Tanaka K, Tian Q et al. 2015 – Towards a natural classification and backbone tree for Lophiostomataceae, Floricolaceae, and Amorosiaceae fam. nov. Fungal Diversity. 74, 199–266.