Pleosporales » Lophiostomataceae » Lophiotrema

Lophiotrema vagabundum

Lophiotrema vagabundum (Sacc.) Sacc., Michelia 1(no. 3): 338 (1878)

Index Fungorum number: IF 210555; Facesofungi number: FoF 2783

Saprobic on dead stem of Sambucus ebulus L. Sexual morph: Ascomata 489–614 μm high × 622–801 μm diam. (x̄ = 551 × 762 μm, n = 5), semi-immersed to erumpent through host tissue, under to clypeus, solitary, uniloculate, globose, conspicuous at the surface, black, subcarbonaceous, without hairs, ostiolate, smooth-walled. Peridium 25–41 μm wide, comprising 5–6 layers of hyaline to reddish- brown cells of textura angularis. Hamathecium 1.1–1.9 μm wide, comprising numerous, aseptate, unbranched, anastomosing pseudoparaphyses. Asci 96–129 × 8–10 μm (x̄ = 118 × 9 μm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical, furcate, rounded at the ends, short pedicellate, lacking an ocular chamber. Ascospores 22–30 × 4–7 μm (x̄ = 25 × 5 μm, n = 15), overlapping 1–2-seriate, hyaline, 3-septate, constricted at the second septum, swollen at second cell, conical at the ends, guttulate, surrounding by large mucilaginous sheath, smooth-walled. Asexual morph Undetermined.

            Culture characteristics: Colonies on MEA reaching 4–5 cm diam., after 7 days in the dark at 16 °C, flat to slightly effuse, entire edge, light brown to dark brown, not sporulating in culture.

Material examined: ITALY, near San Benedetto in Alpe, on dead stem of Sambucus ebulus (Adoxaceae), 24 October 2014, Erio Camporesi, IT2196 (MFLU 15-1497, new host record); living culture MFLUCC 15-0081, (HKAS 94608 bis).

GenBank Numbers LSU:KY496737, ITS:KY496757, SSU:KY501125, TEF1:KY514401.

Notes: Lophiotrema vagabundum was introduced by Saccardo (1878), based on a sexual morph and was recorded from Vitis coignetiae, Epilobium angustifolium and woody plants. Based on rDNA phylogenetic analyses, the collection of L. vagabundum in this study (MFLUCC 15-0081) clustered with high statistical support to other L. vagabundum strains analyzed. Morphological characters are also identical to other L. vagabundum strains and therefore we report this collection as a new host record on Sambucus ebulus (Adoxaceae), from Italy (Tibpromma et al. 2017).