Pseudodidymosphaeria phlei
Pseudodidymosphaeria phlei Phukhams., Camporesi & K.D. Hyde, in Li et al., Fungal Diversity 78: 10.1007/s13225-016-0366-9, [57] (2016)
Index Fungorum number: IF 551895; Facesofungi number: FoF 01816
Etymology: Names base on the host.
Saprobic on dead stem of Phleum pretense L. Sexual morph: Ascomata 200–368 μm diam. (x̄ = 290.7 μm, n = 15), solitary, scattered or gregarious on host, semi-immersed to superficial, globose to subglobose, base flattened, slightly tapering to apex, lacking ostioles. Peridium 9–24 μm wide, composed of 2–3 wall layers, outer layer of light brown to dark brown cells of textura prismatica, inner layer, 1–2 thin gelatinous layers. Hamathecium comprising numerous, long, 2–5 μm wide (x̄ = 2.5 μm, n = 50), transversely septate, branched, cellular pseudoparaphyses, embedded in a gelatinous matrix. Asci 60–100 × 10–20 μm (x̄ = 73.49 × 13.86 μm, n = 20), 8-spored, bitunicate, fissitunicate, clavate to sub-cylindrical, short pedicellate, ocular chamber clearly visible when immature. Ascospores 15–21 × 6–10 μm (x̄ = 16.8 × 7.5 μm, n = 50) bi-seriate or overlapping, ovoid to sub-oval, slightly narrow at the apex, 1-transversely septate, slightly constricted at the septa, mucilaginous sheath clearly visible, immature spores hyaline, light brown to brown when mature, smooth-walled. Asexual morph Undetermined.
Culture characteristics: Ascospore geminating on PDA within 48 h, germ tubes developed from both ends of the ascospores. Colonies on PDA reaching 30 mm diam. after 4 weeks. Culture incubated at 16 °C, at first white, after 2 weeks pale green from center and bottom of colonies. After four weeks olive-green. Colonies morphology, umbonate, with dense mycelium, slightly papillate on the surface, circular, with dentate margin.
Material examined: ITALY, Forlì-Cesena Province, Monte Fumaiolo – Verghereto, on a dead stem of Phleum pretense (Poaceae), 31 July 2014, E. Camporesi (MFLU 15–3281, holotype; isotype HKAS 91937), ex-type living culture, MFLUCC 14–1061, KUMCC 15–0551.
Notes: Pseudodidymosphaeria phlei is introduced from vertical dead stems of Phleum pretense L. (Poaceae). Pseudodidymosphaeria phlei is closely related to the type species, P. spartii (Fabre) Thambugala et al., as in phylogenetic analysis they form sister clades with high support values (100 % ML). Pseudodidymosphaeria phlei nevertheless is distinct in having semi-immersed to superficial ascomata, larger peridium cell walls, with 2–3 wall layers, and ascospores with less distinctly rounded ends. Therefore, we introduce Pseudodidymosphaeria phlei as a new species (Li et al. 2016).
Figure x. Pseudodidymosphaeria phlei (holotype) a, b Appearance of ascomata on host surface c Section throught ascoma on host d Section of peridum e Hyaline cellular pseudoparaphyses f Immature asci g–h Mature asci i–l Ascospores m Ascospores stained in Indian ink to show sheath. Scale bar: b = 200 μm, c = 100 μm, d = 50 μm, e–h, m = 20 μm, i–l = 10 μm.
References:
Li GJ, Hyde KD, Zhao RL, Hongsanan S et al. 2016 – Fungal diversity notes 253–366: taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity 78, 1–237.
Second Record
Pseudodidymosphaeria phlei Phukhams., Camporesi & K.D. Hyde, in Li et al., Fungal Diversity 78: 10.1007/s13225-016-0366-9, [57] (2016)
Index Fungorum number: IF 551895; Facesofungi number: FoF 2786
Etymology: refers to the country where the holotype was collected.
Holotype: MFLU 15-1254
Saprobic on species of Arundo donax L., in terrestrial environments. Sexual morph: Ascomata 300–500 μm high × 250–450 μm diam. (x̄ = 184.6 × 146.2 μm, n = 5), solitary, immersed, coriaceous, dark brown to black, globose to subglobose. Peridium 7–12 μm wide at the base, 12–20 μm wide at the sides, two-layered, and outer layer composed of dark brown, thin-walled cells of textura angularis, inner layer composed of smaller, compressed, hyaline cells. Hamathecium of 1.5–2.5 μm wide, septate, hyaline, cellular pseudoparaphyses, embedded in gelatinous matrix between and above the asci. Asci 100–130 × 12–20 μm (x̄ = 112.9 × 15.1 μm, n = 15), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, with a short pedicel, narrowly rounded at the apex, with an indistinct ocular chamber. Ascospores 19–22 × 9–11 μm (x̄ = 20.2 × 10.2 μm, n = 40), uniseriate to obliquely uniseriate, slightly overlapping, hyaline when immature, becoming golden brown when mature, ellipsoid, with broadly obtuse ends, 1-septate, upper cell slightly broader than the lower one, constricted at the septum, verrucose, surrounded by a thick mucilaginous sheath. Asexual morph: Undetermined.
Culture characteristics: Colonies on PDA growing slowly, reaching 25–30 mm diam. after 21 days at 16 °C, surface greenish-brown, spreading with moderate aerial mycelium, and uneven, smooth margins; reverse buff.
Material examined: ITALY, Forlı`-Cesena, Ravaldino in Monte, on dead Arundo donax stem (Poaceae), 11 November 2014, Erio Camporesi, IT 2233 (MFLU 15-1360, new host record)
GenBank Numbers LSU:KY264748, ITS:KY264744, SSU:KY264752.
Notes: Li et al. (2016) introduced Pseudodidymosphaeria phlei from Phleum pretense L. (Poaceae) collected in Forlı`-Cesena Province of Italy as the second species of Pseudodidymosphaeria. Pseudodidymosphaeria phlei is similar to type species, P. spartii, in having globose to subglobose ascomata, cylindric-clavate asci and 1-septate ascospores. Our new isolate is also morphologically similar to P. phlei (strain MFLUCC 14-1061) and phylogenetically groups with 100% ML support. However, it is associated with a different host. Pseudodidymosphaeria phlei (holotype) was recorded on Phleum pretense and our collection was found on Arundo donax (Poaceae) (Tibpromma et al. 2017).
Figure x. Pseudodidymosphaeria phlei (MFLU 15-1360). a Appearance of ascomata on the host substrate. b Section of ascoma. c Section of peridium. d Pseudoparaphyses. e–g Asci. g–l Ascospores. Scale bars a = 500 μm, b = 100 μm, c = 20 μm, d = 10 μm, e, f = 20 μm, h–l = 10 μm.
References:
Tibpromma S, Hyde KD, Jeewon R, Maharachchikumbura SS et al. 2017 – Fungal diversity notes 491–602: taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity 83, 1-261.
Last Update: 11 December 2021