Muriformistrickeria rosae
Muriformistrickeria rosae Wanas., Camporesi, E.B.G. Jones & K.D. Hyde, in Wanasinghe et al., Fungal Diversity: 10.1007/s13225-018-0395-7, [96] (2018)
Index Fungorum number: IF 554164; Facesofungi number: FoF 04005
Etymology: The specific epithet reflects the host genus Rosa.
Holotype: MFLU 16-0227.
Saprobic on dead spines of Rosa. Sexual morph: Ascomata 150–220 μm high, 300–350 μm diam. (x̄ = 198.8 × 325.8 μm, n = 5), superficial, solitary, scattered, broadly oblong and flattened, dark brown to black, coriaceous, ostiolate. Ostiole 30–50 μm high 40–70 μm diam. (x̄ = 42.3 × 54.7 μm, n = 5), black, ostiolar canal filled with hyaline cells. Peridium 15–25 μm wide at the base, 25–40 μm wide at the sides, two layers, outer layer heavily pigmented, thick-walled, comprising reddish to dark brown cells of textura angularis, inner layer composed of hyaline thick-walled cells of textura angularis. Hamathecium comprising numerous, 2–3 μm wide, filamentous, branched, septate, pseudoparaphyses. Asci 70–100 × 11–17 μm (x̄ = 93.9 × 13.8 μm, n = 40), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, pedicellate, thick walled at the apex, with minute ocular chamber. Ascospores 15–23 × 6–10 μm (x̄ = 19.3 × 8.2 μm, n = 50), overlapping 1–2-seriate, muriform, ellipsoidal to subfusiform, slightly curved, upper part wider than the lower part, 3–4-transversely septate, with 1 vertical septum, deeply constricted at the central septum, hyaline, with rounded ends, without a mucilaginous sheath. Asexual morph: Undetermined.
Material examined: ITALY, Arezzo Province, near Ortignano, on dead aerial spines of Rosa canina (Rosaceae), 29 September 2014, Erio Camporesi IT 2129 (MFLU 16-0227, holotype).
GenBank Numbers: ITS: MG828918, LSU: MG829028, SSU: MG829135, TEF: MG829215.
Notes: Muriformistrickeria rosae clusters with M. rubi with high support. Despite this phylogenetic relatedness, we consider them as different species. Both species have been collected from Italy, but from different hosts: M. rosae from Rosa; M. rubi from Rubus sp. Morphologically, these two-species are similar in ascomatal characters with muriform ascospores. However, they differ from each other based on other ascospore characteristics; Muriformistrickeria rosae has hyaline, subfusiform, slightly curved ascospores with 3–4 transverse septa and with 1 vertical septum, while M. rubi has mostly ellipsoidal, ascospores with 4–6 transverse septa and 1–2 vertical septa, which are initially light yellow, becoming yellowish-brown at maturity. We did not obtain an isolate and therefore we isolated DNA directly from the fruiting bodies.
Figure x. Muriformistrickeria rosae (MFLU 16-0227, holotype). a Ascomata on host substrate. b Section of ascoma. c Peridium. d Pseudoparaphyses. e–g Asci. h–l Ascospores. Scale bars: b = 100 μm, c = 50 μm, e–g = 20 μm, d, e–i = 10 μm.
References:
Wanasinghe DN, Phukhamsakda C, Hyde KD, Jeewon R et al. 2018 – Fungal diversity notes 709–839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal diversity 89, 1–236.