Dematiopleospora cirsii
Dematiopleospora cirsii Wanas., Camporesi, E.B.G. Jones & K.D. Hyde, in Hyde et al., Fungal Diversity 2015: [89] (2015) [2016]
Index Fungorum number: IF 552135; Facesofungi number: FoF 02205
Etymology: Name reflects the host genus Cirsium, from which the species was collected.
Holotype: MFLU 16-0145.
Saprobic on dead and hanging branches of Cirsium sp. Sexual morph: Ascomata 250–300 μm high 250–350 μm diam. (x̄ = 271.1 × 299.5 μm, n = 5), immersed to semi-erumpent, solitary, scattered, broadly oblong and flattened, dark brown to black, coriaceous, cupulate when dry, ostiolate. Ostiole 50–65 μm high 40–50 μm diam. (x̄ = 58.2 × 43.4 μm, n = 5), papillate, black, smooth, with short and light brown setae. Peridium 10–20 μm wide at the base, 15–30 μm wide in sides, thick, with 4–5 layers, outer layer heavily pigmented, thick-walled, comprising reddish to dark brown cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis. Hamathecium comprising numerous, 1.5–2 μm wide, filamentous, branched, septate, pseudoparaphyses. Asci 80–120 × 10–14 μm (x̄ = 100.9 × 12.3 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical to cylindricclavate, pedicellate, thick-walled at the apex, with minute ocular chamber. Ascospores 20–30 × 6–9 μm (x̄ = 25.9 × 6.6 μm, n = 50), overlapping uni-seriate, muriform, ellipsoidal to subfusiform, slightly curved, upper part wider than the lower part, 6–7 transversely septate, with 1 vertical septum, deeply constricted at the central septum, initially hyaline, becoming golden-brown at maturity, ends remaining cone-shaped, with rounded ends, without a mucilaginous sheath. Asexual morph: Undetermined.
Culture characteristics: Colonies on MEA, reaching 1.5 cm diam. after 30 days at 18 °C, circular, smooth margin white at first, greenish-grey after 4 weeks, flat on the surface, without aerial mycelium, reverse blackish green. Hyphae septate, branched, hyaline, thin, smooth-walled.
Material examined: ITALY, Arezzo [AR] Province, Papiano, dead herbaceous branches of Cirsium sp. (Asteraceae), 13 June 2013, E. Camporesi (MFLU 16-0145, holotype); (isotype in BBH), ex-type living culture, MFLUCC 13-0615.
GenBank:
Notes: In this study of Hyde et al. (2016), the strain of MFLUCC 13-0615, groups with Dematiopleospora mariae (type strain of Dematiopleospora) with high bootstrap and Bayesian support. Dematiopleospora cirsii resembles D. mariae in having brown periphyses in the ostiole, a peridium comprising brown to dark brown cells of textura angularis and multi-septate ascospores, but differs in having immersed ascomata, ascospores with 7 transverse septa and much longer lower part than upper part, while D. mariae has superficial ascomata, ascospores with 9–10 transverse septa, and upper and lower parts of equal length.
Figure x. Dematiopleospora cirsii (holotype). a Appearance of ascomata immersed beneath host substrate. b, c Sections of ascomata. d Close up of ostiole with short, light brown, setose hyphae. e Peridium. f Pseudoparaphyses. g–j Asci. k–n Ascospores. o Germinated ascospore. p, q Culture on PDA (note q reverse). Scale bars a = 500 μm, b = 100 μm, c, d = 50 μm, e, k–o = 10 μm, f = 5 μm, g–j = 20 μm.
References:
Hyde KD, HongsananS, Jeewon R, Bhat DJ et al. 2016 – Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity 80, 1–270.
Last Update: 06 May 2021