Dematiopleospora rosicola
Dematiopleospora rosicola Wanas., Camporesi, E.B.G. Jones & K.D. Hyde, in Wanasinghe et al., Fungal Diversity: 10.1007/s13225-018-0395-7, [115] (2018)
Index Fungorum number: IF 554177; Facesofungi number: FoF 04019
Etymology: The specific epithet reflects the host genus Rosa.
Holotype: MFLU 16-0232.
Saprobic on dead spines of Rosa canina. Sexual morph: Ascomata 150–250 μm high, 200–300 μm diam. (x̄ = 193.5 × 229.4 μm, n = 5), immersed to erumpent, solitary, scattered, globose or subglobose, dark brown to black, coriaceous, ostiolate. Ostiole 40–50 μm high, 20–30 μm diam. (x̄ = 44.5 × 26.7 μm, n = 5), papillate, black, smooth, comprising short, dark brown setae. Peridium 7–15 μm wide at the base, 10–16 μm wide at the sides, comprising heavily pigmented, thin-walled, reddish to dark brown cells of textura angularis. Hamathecium comprising numerous, 2.5 lm wide, filamentous, branched, septate, pseudoparaphyses. Asci 80–90 × 15–17 μm (x̄ = 84.8 × 16.1 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindric-clavate to clavate, pedicellate, thick-walled at the apex, with minute ocular chamber. Ascospores 18–22 × 7–10 μm (x̄ = 21.6 × 8.6 μm, n = 50), overlapping 1–2-seriate, muriform, ellipsoidal to subfusiform, slightly curved, upper part wider than the lower part, 4–7-transversely septate, with 1 vertical septum, constricted at the septa, initially hyaline, becoming yellowish-brown at maturity, ends remaining lighter and cone-shaped, with rounded ends, wall firm, smooth, lacking mucilaginous sheath. Asexual morph: Undetermined.
Material examined: ITALY, Province of Forli-Cesena [FC], Alfero, Bagno di Romagna, on dead aerial spines of Rosa canina L. (Rosaceae), 24 October 2014, Erio Camporesi IT 2199 (MFLU 16-0232, holotype).
GenBank Numbers: ITS: MG828888, LSU: MG829006, SSU: MG829117.
Notes: The ascospores of this fungus failed to germinate and grow in culture. Therefore, we have extracted DNA directly from fruiting bodies. Dematiopleospora rosicola and D. cirsii are closely related in the phylogenetic analyses. They are similar in their morphological features except ascospore dimensions. Dematiopleospora cirsii (20–30 μm) has comparatively longer ascospores than D. rosicola (18–22 μm). To further support the establishment of the new taxon as proposed by Jeewon and Hyde (2016), we examined the nucleotide differences within the ITS regions (ITS1- 5.8S-ITS2 gene region. Comparison of the 575 nucleotides across the ITS regions reveals 13 bp (2.3%) differences between Dematiopleospora rosicola and D. cirsii.
Figure x. Dematiopleospora rosicola (MFLU 16-0232, holotype). a Ascomata on host substrate. b Section of ascoma. c Ostiole. d Peridium. e Pseudoparaphyses. f–h Asci. i-n Ascospores. Scale bars: b = 100 μm, c–h = 20 μm, i–n = 10 μm.
References:
Jeewon R, Hyde KD (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7, 1669–1677.
Wanasinghe DN, Phukhamsakda C, Hyde KD, Jeewon R et al. 2018 – Fungal diversity notes 709–839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal diversity 89, 1–236.