Pleosporales » Phaeosphaeriaceae » Neosetophoma

Neosetophoma camporesii

Neosetophoma camporesii Q. Tian & K.D. Hyde, in Hyde et al., Fungal Diversity 100: 99 (2020)

Index Fungorum number: IF556934; Facesofungi number: FoF 06859

            Etymology: Named in honor of Mr. Erio Camporesi who made an immense contribution to fungal investigation and collection.

Holotype: MFLU 153394

Saprobic on leaves of Rosa sp. Sexual morph: Ascomata 235–360 μm high × 170–280 μm diam. (x̄ = 295 × 220 μm, n = 10), scattered or sometimes gregarious, globose, subglobose to turbinate, coriaceous, black, smooth, ostiolate. Ostiole fat, epapillate, with a reddish tinge at the pore. Peridium thick at apex, 15–26 μm thick (x̄ = 23 μm, n = 10) at the sides, 2-layered, outer layer composed of irregular, thick-walled, brown to dark brown cells of textura epidermoidea, cells up to 3 μm diam., inner layer composed of small, thin-walled, pale brown cells, up to 5 μm diam. Hamathecium of dense, 1–2 μm broad, long, pseudoparaphyses, embedded in mucilage, anastomosing between and above asci. Asci 62–90 × 7–10 μm (x̄ = 84 × 8.5 μm, n = 10), 8-spored, bitunicate, cylindro-clavate to clavate, with a short pedicel. Ascospores 2-seriate above and 1-seriate or overlapping 1-seriate below, 20–32 μm × 3–5 μm (x̄ = 27 × 4.2 μm, n = 10), hyaline to pale brown, fusoid, obtuse at the apex and tapering towards the lower end cell, mostly straight, sometimes slightly curved, 3-septate, slightly constricted at the septa, smooth-walled. Asexual morph: Undetermined.

Culture characteristics: Ascospores germinating on water agar and germ tubes produced from ascospores within 12 h. Colonies on PDA reaching 40 mm in 7 days at 25 °C, margin feathery. Mycelium aerial, white, becoming green to yellow towards the edge from above. No asexual morph was produced on PDA after 60 days incubation.

Material examined: ITALY, Province of Forlì-Cesen [FC], Pian di Spino-Civitella di Romagna, on dead spines of Rosa canina L. (Rosaceae), 3 February 2015, E. Camporesi, IT2361 (MFLU 15-3394, holotype; KUN-HKAS 92414, isotype), ex-type living cultures, MFLUCC 15-0682, KUMCC 15-0080.                

GenBank Numbers: ITS: KU302779, LSU: KU302778, SSU: MN653132, TEF1-α : MN654114.

Note: Based on a mega blast search using the ITS sequence, the closest matches in NCBI’s GenBank nucleotide database were Neosetophoma shoemakeri (GenBank NR_161044; Identities 524/529 (99%), no gap), N. clematidis (GenBank LC171715; Identities 523/530 (99%), no gaps). The highest similarities using the LSU sequence were Coniothyrium cerealis (GenBank MH873258; Identities 878/879 (99%), no gaps), Neosetophoma shoemaker (GenBank MG844348; Identities 878/879 (99%), no gaps). The highest similarities using the SSU sequence were Poaceicola agrostina (GenBank NG_062198; Identities 1004/1004 (100%), no gaps). The highest similarities using the TEF1-α sequence were Neosetophoma shoemakeri (GenBank MG844352; Identities 855/873 (98%), no gaps).

The sexual morph taxon, Neosetophoma camporesii, is introduced based on phylogenetic analyses of combined ITS, LSU, SSU and TEF1-α sequence data, and distinct morphological characters. Phylogenetically, our isolate formed a distinct clade within Neosetophoma in Phaeosphaeriaceae distant from other four sexual morph species (MFLUCC 140528, MFLU161606, GZ13 and GZAAS 180100). Morphologically, N. camporesii is similar to Paraleptosphaeria nitschkei, but differs in its 3-septate, fusoid ascospores (obtuse at the apex and tapering at the end). Neosetophoma guiyangensis and N. xingrensis formed sister relationships in phylogenetic analysis with high bootstrap support. Both species share the similar characters of fusiform, hyaline to pale brown guttulate ascospores, but N. guiyangensis has normally 3–5-septate ascospores and a furcate pedicel, while N. xingrensis has 1–3-septate ascospores and the pedicel is sessile (Hyde et al. 2018). However, the most conspicuous difference between these four taxa is that N. camporesii has a clear ascomatal ostiole with a reddish tinge at the pore.