Pleosporales » Phaeosphaeriaceae » Nodulosphaeria

Nodulosphaeria multiseptata

Nodulosphaeria multiseptata Tibpromma, Camporesi & K.D. Hyde, in Tibpromma et al., Fungal Diversity 83: 91 (2017)

Index Fungorum number: IF 552700; Facesofungi number: FoF 2791

            Etymology: refers to the ascospore morphology.

Holotype: MFLU 14-0720

Saprobic on dead stem of Sambucus ebulus L. Sexual morph: Ascomata 232–260 μm high × 267–272 μm diam. (x̄ = 246 × 269 μm, n = 5), immersed to semi-immersed, solitary, uniloculate, globose to subglobose, black, smoothwalled. Ostiole 60–74 × 83–102 μm (x̄ = 67 × 96 μm, n = 5), papillate, protruding from the centre of ascomata, with numerous, short, internal brown to dark brown setae. Peridium 19–27 μm wide, comprising of dark brown cells of textura angularis. Hamathecium 3.3–4.5 μm wide, comprising numerous, aseptate, distinctly guttulate, pseudoparaphyses. Asci 74–127 × 17–21 μm (x̄ = 100 × 19 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindric-clavate, with short, club-shaped pedicel, thick-walled at the apex, with a minute ocular chamber. Ascospores 56–70 × 5–6 μm (x̄ = 65 × 6 μm, n = 20), overlapping 1–2-seriate, hyaline when young, becoming golden yellow at maturity, fusiform, 12–14-septate, slightly constricted at the septum, enlarged at fifth cell from apex, conical and narrowly rounded at the ends, guttulate, with mucilaginous appendages at the ends. Asexual morph Undetermined.

           Culture characteristics: Colonies on MEA reaching 8 cm diam. after 7 days at 16 °C, whitish-gray, cottony, dense, with sparse aerial mycelium on the surface.

Material examined: ITALY, Province of Arezzo, near Ortignano-Raggiolo, on dead stem of Sambucus ebulus (Adoxaceae), 25 May 2014, Erio Camporesi, IT1889 (MFLU 14-0720, holotype); ex-type living culture MFLUCC 15-0078 (HKAS 94591, paratype)

GenBank Numbers LSU:KY496728, ITS:KY496748, SSU:KY501116, TEF1:KY514396.

Notes: In the phylogenetic analysis N. multiseptata clustered with N. guttulatum with high statistical support (90% in ML, 0.98 in BYPP), but the species are different. Nodulosphaeria guttulatum has 4-septate, fusiform ascospores, enlarged at the second cell from the apex, with conical and narrowly rounded ends (Tibpromma et al. 2017).