Nodulosphaeria sambuci
Nodulosphaeria sambuci Tibpromma, Camporesi & K.D. Hyde, in Tibpromma et al., Fungal Diversity 83: 94 (2017)
Index Fungorum number: IF 552701; Facesofungi number: FoF 2792
Etymology: named for its occurrence on the host plant.
Holotype: HKAS94536
Saprobic on dead stem of Sambucus ebulus L. Sexual morph: Ascomata 204–220 μm high × 173–215 μm diam. (x̄ = 213 × 198 μm, n = 5), superficial, solitary, or in small groups, uniloculate, globose to subglobose, conspicuous at the surface, black, hairy. Ostioles 78–92 × 107–126 μm (x̄ = 86 × 117 μm, n = 5), papillate, protruding from the centre of the ascomata, with numerous, short, brown to dark brown setae. Peridium 23–44 μm wide, comprising of light-brown to dark brown cells of textura angularis. Hamathecium 1.1–3.4 μm wide, comprising dense, numerous, aseptate, distinctly guttulate pseudoparaphyses. Asci 53–80 × 7–10 μm (x̄ = 68 × 9 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindricclavate, with small furcate pedicel, apically rounded, with a minute ocular chamber. Ascospores 32–35 × 3–5 μm (x̄ = 34 × 4 μm, n = 15), overlapping 1–2-seriate, hyaline when young, becoming golden yellow at maturity, fusiform, 8-septate, slightly constricted at the septum, enlarged at four cell, conical and narrowly rounded at the ends, guttulate, with small mucilaginous appendages at both ends. Asexual morph: Undetermined.
Culture characteristics: Colonies on MEA reaching 4cm diam. after 7 days at 16 °C, gray to dark, cottony, flat, dense, with sparse aerial mycelium on the surface.
Material examined: ITALY, Province of Forlı`-Cesena, Monte Fumaiolo, on dead stem of Sambucus ebulus (Adoxaceae), 10 July 2012, Erio Camporesi, IT558 (HKAS 94536, holotype); ex-type living culture MFLUCC 15-0068.
GenBank Numbers LSU:KY496721, ITS:KY496742, SSU:KY501112.
Notes: Nodulosphaeria sambuci shares similarities with species having 8-septate ascospores (Nodulosphaeria derasa, N. dolioloides and N. pellita), but those species lack molecular data. It is phylogenetically closely related to N. senecionis with high statistical support (100% in ML, 1.00 in BYPP). However, N. sambuci has fusiform, golden yellow ascospores, that are enlarged at the fourth cell from the apex, and conical and narrowly rounded at the ends, while N. senecionis has long fusiform to almost cylindrical, yellowish brown ascospores, enlarged at the third cell from the apex and tapering towards the rounded ends (Ariyawansa et al. 2015c).
(taken from Tibpromma et al. 2017).
Figure x. Nodulosphaeria sambuci (HKAS94536, holotype). a Appearance of ascomata on host substrate. b Section of ascoma. c, d Section of ostiole. e Section of peridium. f Pseudoparaphyses. g, h Asci. i–k Ascospores. l Germinating ascospore. Scale bars a = 100 μm, b = 50 μm, c–e = 20 μm, f = 5 μm, g, h = 20 μm, i–l = 10 μm.
References:
Tibpromma S, Hyde KD, Jeewon R, Maharachchikumbura SS et al. 2017 – Fungal diversity notes 491–602: taxonomic and phylogenetic contributions to fungal taxa. Fungal diversity 83, 1-261.
Last update: 04 July 2021