Nodulosphaeria scabiosae
Nodulosphaeria scabiosae Mapook, Camporesi & K.D. Hyde, in Mapook, Boonmee, Ariyawansa, Tibpromma, Campesori, Gareth Jones, Bahkali & Hyde, Mycol. Progr. 15(no. 34): 19 (2016)
Index Fungorum number: IF 551943; Facesofungi number: FoF 01893
Etymology: Name reflects the host genus Scabiosa, from which this species was collected.
Holotype: MFLU 15–1129
Saprobic on dead stems of Scabiosa sp., noticeable as black dots on host surface. Sexual morph: Ascomata (195–)200–210(–236) × (192–)215–232(–265) μm (x̅ = 210 × 225 μm, n = 5), immersed, erumpent at maturity, solitary, scattered, subglobose to obpyriform, coriaceous, dark brown, without a subiculum covering the host. Ostiole papillate, protruding from the substrate, with numerous, external brown to dark brown setae. Peridium 25–30 μm wide, comprising 4–5 layers of dark brown cells of textura angularis. Hamathecium comprising 2.5–3 μm wide, filiform, septate, branching pseudoparaphyses. Asci 105–125 × 11–13 μm (x̅=115× 12.5 μm, n =10), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-subclavate, slightly curved, short pedicellate, apically rounded, with an ocular chamber. Ascospores 35–40 × (3–)5–6 μm (x̅ = 37 × 5 μm, n = 20), overlapping 2–3-seriate, hyaline or pale yellowish brown, cylindric-fusiform, 4-septate, tapering towards the rounded ends, enlarged at the second cell from the apex, constricted at septum, straight to slightly curved, guttulate, lacking terminal appendages. Asexual morph: Undetermined.
Culture characteristics: Ascospores germinating on MEA within 36 hr. at 18 °C and germ tubes produced from both ends.
Material examined: ITALY, Forlì-Cesena Province, Fiumicello – Premilcuore, on dead stems of Scabiosa sp. (Caprifoliaceae), 3 June 2014, E. Camporesi (MFLU 15–1129, holotype), ex-type living culture, MFLUCC 14–1111, MUCL; (isotype in HKAS, under the code of HKAS 90973); on dead stem of Scabiosa sp. (Caprifoliaceae), 16 June 2014, E. Camporesi (MFLU 15–1878, paratype)
GenBank:
Figure x. Nodulosphaeria scabiosae (holotype). a Appearance of immersed ascomata on host substrate. B Section through ascoma. c Ostiole with external dark brown setae. d Peridium. E Pseudoparaphyses. f– i Immature and mature asci. j–o Ascospores. Scale bars (b, f–i) 50 μm, (j–o) 20 μm, (c) 10 μm, (d, e) 5 μm.
References:
Mapook A, Boonmee S, Ariyawansa HA, Tibpromma S, Campesori E et al. 2016 – Taxonomic and phylogenetic placement of Nodulosphaeria. Mycological Progress 15, 34.
Last update: 22 July 2021