Comoclathris rosae
Comoclathris rosae Wanas., Camporesi, E.B.G. Jones & K.D. Hyde, in Wanasinghe et al., Fungal Diversity: 10.1007/s13225-018-0395-7, [149] (2018)
Index Fungorum number: IF 554204; Facesofungi number: FoF 04043
Etymology: The specific epithet reflects the host genus Rosa.
Holotype: MFLU 15-0203.
Saprobic on Rosa canina. Sexual morph: Ascomata 120–150 μm high 175–200 μm diam. (x̄ = 135.7 × 173.7 μm, n = 5), immersed to erumpent, globose or subglobose, dark brown to black, coriaceous. Peridium 8–15 lm wide at the base, 10–15 μm wide at the sides, comprising reddish to dark brown cells of textura angularis. Hamathecium comprising numerous, 2 lm wide, filamentous, branched, septate, pseudoparaphyses. Asci 70–110 × 15–30 μm (x̄ = 87.4 × 19.7 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindric-clavate to clavate, pedicellate, thick-walled at the apex, with minute ocular chamber. Ascospores 20–30 × 8–15 μm (x̄ = 24.9 × 11.3 μm, n = 50), overlapping 1–2-seriate, mostly ellipsoidal, muriform, 4–7-transversely septate, with 1–2 vertical septa, deeply constricted at the middle septum, slightly constricted at the remaining septa, initially hyaline to pale yellow, becoming pale brown at maturity, conically rounded at both ends, surrounded by a thick mucilaginous sheath. Asexual morph: Undetermined.
Material examined: ITALY, Arezzo Province, Bagno di Cetica, on dead aerial spines of Rosa canina L. (Rosaceae) 10 April 2015, Erio Camporesi IT 2183 (MFLU 15-0203, holotype). ITALY, Province of Forli-Cesena [FC], near Monte Fumaiolo, on dead aerial spines of Rosa canina L. (Rosaceae), 27 October 2014, Erio Camporesi IT 2209 (MFLU 16-0234).
GenBank Numbers: ITS: MG828876, MG828877, LSU: MG828992, MG828993, SSU: MG829103, MG829104, RPB2: MG829249, MG829250.
Notes: During the study of saprobic fungi in Italy, two isolates recovered from Rosa canina were morphologically similar in their ascomata, pseudoparaphyses, asci and ascospore characteristics. The dimensions of ascospores and asci were almost identical and in our phylogenetic analyses, these two isolates group in a monophyletic clade within Comoclathris and sister to C. lini and C. sedi. The ascospore dimensions of our new isolates (20–30 × 8–15 μm) are comparatively larger than C. sedi (19–20 × 8–10 μm). The ascospores of our new isolates differ from C. lini in having conically rounded ends, while C. lini has broadly rounded ends. Therefore, we introduce our new isolates as Comoclathris rosae sp. nov. We did not obtain an isolate and therefore we isolated DNA directly from the fruiting bodies.
Figure x. Comoclathris rosae (MFLU 16-0234, holotype). a Ascomata on host substrate. b Section of ascoma. c Peridium. d Pseudoparaphyses. e, f Asci. g–k Ascospores. l Ascospore stained with Indian ink showing sheath. Scale bars: b = 50 μm, c, d = 10 μm, e, f = 20 μm, g–l = 10 μm.
References:
Wanasinghe DN, Phukhamsakda C, Hyde KD, Jeewon R et al. 2018 – Fungal diversity notes 709–839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal diversity 89, 1–236.