Anthocanalis spartii
Anthocanalis spartii Daranag., Camporesi & K.D. Hyde [as 'sparti'], in Daranagama, Camporesi, Tian, Liu, Chamyuang, Stadler & Hyde, Fungal Diversity 73: 211 (2015)
Index Fungorum number: IF 820973; Facesofungi number: FoF 00202
Saprobic on stems, branches and twigs. Sexual morph: Ascomata 250–300 μm diam. and 250–270 μm high, immersed, solitary and scattered, appearing as raised black spots, in cross section, conical to globose, with poorly developed clypeus and shiny papilla. Peridium 30–35 μm diam. at base, comprising two cell layers, outwardly comprising thick-walled, light brown cells of textura irregularis and inwardly comprising thin-walled, hyaline cells of textura angularis. Paraphyses 5 μm diam., numerous, filamentous, septate, slightly longer than the asci. Asci 100–150 × 5–8 μm, 8-spored, unitunicate, cylindrical, short-pedicellate, with rectangular to discoid, 1.5–2.5 μm high × 0.5–1 μm diam., J+, apical ring, bluing in Melzer’s reagent. Ascospores 15–18 × 6–8.5 μm, overlapping uniseriate, unicellular, limoniform, with one side flattened to concave, dark brown, smooth-walled, mucilaginous sheath clearly visible at the flattened side, sheath 1–2 μm thick, constricted at centre along the flattened edge forming an indentation, germ slit, full length, straight. Asexual morph: Conidiophores up to 50–80 μm long and 1.5–2 μm diam., complex, nodulisporium-like, hyaline, branched. Conidiogenous cells 10–12 μm long and 1.5–1.8 μm diam., terminal at the branches, cylindrical. Conidia 5–5.5 × 2–2.5 μm, hyaline, ellipsoidal, aseptate, unicellular.
Material examined: ITALY, Province of Forlı`-Cesena, Corniolo-Santa Sofia, on Spartium junceum L. (Fabaceae), 25 Oct. 2012, Erio Camporesi IT848 (MFLU 14–0225, holotype), ex-type culture, MFLUCC 14–0010 = ICMP; Ibid. 25 Oct. 2012, Erio Camporesi IT848-bis (PDD, isotype); Monte Riccio, Bagno di Romagna, 20 Dec. 2013, Erio Camporesi IT848–tris (MFLU 14–0226, PDD paratype), ex-paratype culture, MFLUCC 14–0557.
GenBank:
Notes: Anthocanalis sparti was introduced by Daranagama et al. (2015) and illustrated in detail from both morphological and phylogenetic perspectives. Anthocanalis sparti contains a nodulisporium-like asexual morph and in the four-DNA loci phylogenetic analysis clustered in a monophyletic group with close relationship to Daldinia-like stromatic taxa. Anthostomella colligata K.D. Hyde & B.S. Lu, A. flagellariae (Rehm) B.S. Lu & K.D. Hyde and A. francisiae K.D. Hyde, which also possess immersed ascomata short-pedicellate asci and ascospores with constricted mucilaginous sheaths, have close morphological resemblance to A. sparti. Thus, it may be wise to transfer these taxa from the hyper-diverse, large genus of Anthostomella into Anthocanalis following molecular study.
Figure x. Anthocanalis sparti (holotype). a Habit. b Ascomata in wood. c Cross section of an ascoma. d Paraphyses. e, f Asci g Asci in Melzer’s reagent with rectangular to discoid, J+, apical apparatus and straight, full length germ slit (in mature ascospores). h–k Ascospores in water with mucilaginous sheath. Scale bars a = 500 μm, b = 1000 μm, c, d = 50 μm, e–k = 10 μm.
Figure y. Asexual morph of Anthocanalis sparti (culture from holotype) a, b Colony on OA after 4 weeks. a From above. b From below. c–e Development of sporulating regions after 7, 14 and 21 days. f Oil droplets. g–i Conidiogenous structures. j, k Conidiogenous cells. l Conidia. m Mycelia. Scale bars g–k = 20 μm, l = 5 μm, k = 30 μm.
References:
Daranagama DA, Hyde KD, Sir EB, Thambugala KM et al. 2018 – Towards a natural classification and backbone tree for Graphostromataceae, Hypoxylaceae, Lopadostomataceae and Xylariaceae. Fungal Diversity 88, 1–65.
Last update: 01 August 2021