Alloanthostomella rubicola
Alloanthostomella rubicola (Speg. ex Sacc. & Trotter) Daranag., Camporesi & K.D. Hyde, in Daranagama, Camporesi, Jeewon, Liu, Stadler, Lumyong & Hyde, Cryptog. Mycol. 37(4): 518 (2016)
Basionym: Anthostomella rubicola Speg. ex Sacc. & Trotter 1913
Index Fungorum number: IF 552372; Facesofungi number: FoF 00320
Saprobic on branches of Rubus sp. and Cornus sanguinea L. Sexual morph: Pseudostromata carbonaceous, black, comprising fungal tissues and epidermal host cells. Ascomata 250–300 × 280–320 μm, immersed, visible as blackened, slightly raised, conical, stromata-like areas, in cross section globose, solitary or aggregated into clusters, scattered, with a central black ostiolar dot, disc-like, black. Clypeus 60–83 μm diam. at base, 75–80 μm high, comprising host cells and intracellular fungal hyphae, surrounded by carbonaceous tissues. Peridium 20–30 μm diam., with a few compressed cell layers, outwardly comprising thick-walled, light to dark brown cells of textura irregularis and inwardly comprising thin-walled, hyaline cells of textura angularis. Paraphyses 5–6 μm diam., filamentous, few, septate. Asci 120–165 × 6–9 μm, 8-spored, unitunicate, cylindrical, short-pedicellate, apically rounded, with a J+ apical ring bluing in Melzer’s reagent, wedge-shaped to cylindrical, 3–5 × 4–5 μm. Ascospores 22–35 × 5.5–8.7 μm, overlapping uniseriate to biseriate, unicellular with rostrate, hyaline dwarf cell, 4–6 μm long, ellipsoidal, with one end tapered and pointed, always remain hyaline in asci, after release from ascus larger cell rarely dark brown or mostly hyaline, smooth-walled. Asexual morph: Undetermined.
Material examined: ITALY, Province of Forlı`-Cesena, Santa Marina in Particeto – Predappio, on Rubus ulmifolius Schott. (Rosaceae), 5 Jan. 2014, Erio Camporesi IT1630 (MFLU 14–0234), living cultures, MFLUCC 14-0175=ICMP; ibid. 5 Jan. 2014, Erio Camporesi IT1630- bis (PDD); Province of Forlı`-Cesena, Trivella di Predappio, on dead branch of Cornus sanguinea (Cornaceae), 26 Dec. 2014, Erio Camporesi, IT 2316 (MFLU 15–0661), living cultures MFLUCC 16–0479, KUMCC.
GenBank:
Notes: Ascospores of Alloanthostomella rubicola remain hyaline in the asci. However, when mature, brown ascospores occur with a hyaline dwarf cell which is rostrate (Lu and Hyde 2000; Daranagama et al. 2015). Attempts to obtain the asexual morph in culture were not successful even after 3 months’ observation. The holotype of A. rubicola collected by Spegazzini, deposited at LPS is from Chile and on Rubus fruticosus L., which was not possible to borrow.
Figure x. Alloanthostomella rubicola (MFLU 15–0661). a, b Pseudostromata on host. c, d Cross section of ascoma. e Peridium. f–i Asci. j Apical ring bluing in Melzer’s reagent. k–m Ascospores. Scale bars a, b = 500 μm, c, d = 100 μm, e = 30 μm, f–j = 10 μm, k–m = 10 μm.
References:
Daranagama DA, Hyde KD, Sir EB, Thambugala KM et al. 2018 – Towards a natural classification and backbone tree for Graphostromataceae, Hypoxylaceae, Lopadostomataceae and Xylariaceae. Fungal Diversity 88, 1–65.
Last update: 28 July 2021