Pseudoanthostomella pini-nigrae
Pseudoanthostomella pini-nigrae Daranag., Camporesi & K.D. Hyde, in Daranagama, Camporesi, Jeewon, Liu, Stadler, Lumyong & Hyde, Cryptog. Mycol. 37(4): 530 (2016)
Index Fungorum number: IF 552250; Facesofungi number: FoF 02390
Saprobic on dead cones of Pinus nigra J.F. Arnold. Sexual morph: Ascomata 250–275 × 330–350 μm, semi-immersed, raised, dome-shaped areas, visible as blackened, black, carbonaceous, solitary, in cross section globose-subglobose, with flattened top. Clypeus black, thick-walled, short, comprising dark fungal hyphae and host epidermal cells. Peridium 17–25 μm wide, with two cell layers, outwardly comprising thick-walled, carbonaceous, compressed, dark brown cells of textura irregularis and inwardly comprising thin-walled, hyaline cells of textura angularis. Paraphyses less than 5 μm wide at base, slightly shorter than the asci, numerous, filamentous, septate. Asci 90–120 × 11–13.5 μm, 8-spored, unitunicate, cylindrical clavate, short pedicellate, sometimes reduced or absent, apically rounded, with J+ apical ring bluing in Melzer’s reagent, discoid-inverted hat-shaped, with thin lower ring, 1.5–2.5 μm high × 4.2–5 μm wide. Ascospores 10–15 × 8.5–12 μm, overlapping uniseriate, unicellular, broadly ellipsoidal, dark brown–black, smooth-walled, with conspicuous mucilaginous sheath, 3–3.4 μm thick, germ slit on ventral side of the ascospore, straight, extending over the full length, sometimes visible as wide horizontal pallid band. Asexual morph: Hyphomycetous. Conidiophores more than 80 μm long and 2.5–3 μm wide, erect, arising from horizontal hyphae, complex, septate, dichotomously branched, smooth, upper region hyaline, lower region light brown. Conidiogenous cells 10–15 μm long and 2–3 μm diam., monoblastic, discrete, terminal on the branches, arising in clusters of 3–4, denticulate. Conidia 6–8.5 × 2–3.5 μm, hyaline, elongated ellipsoidal-fusiform, sometimes curved at apical end, aseptate, smooth-walled.
Material examined: ITALY, Province of Forlı`-Cesena, Montecoronaro – Verghereto, on dead and land cones of Pinus nigra (Pinaceae), 30 July 2014, Erio Camporesi, IT 2027 (MFLU 15–0652, holotype), ibid. (MFLU 15–3274, HKAS 95065, isotypes), ex-type living cultures MFLUCC 16–0478, KUMCC.
GenBank:
Notes: Currently, in updated classification by Wijayawardene et al. (2020) assigned Pseudoanthostomella into Xylariales genera incertae sedis.
Figure x. Pseudoanthostomella pini-nigrae (MFLU 15–3274). a, b Appearance of ascomata on host surface. c Cross section of ascoma. d–f Asci. g Paraphyses. h Apical ring in Melzer’s reagent. i, j Ascospores. Scale bars a–b = 200 μm, c = 100 μm, g = 30 μm, h = 5 μm, i–j = 20 μm.
Figure y. Asexual morph of Pseudoanthostomella pini-nigrae (MFLUCC 16–0478). Culture on OA; a Upper view. b Lower view. c–e Conidiophores. f Conidia. Scale bars c–e = 20 μm, f = 10 μm.
References:
Daranagama DA, Hyde KD, Sir EB, Thambugala KM et al. 2018 – Towards a natural classification and backbone tree for Graphostromataceae, Hypoxylaceae, Lopadostomataceae and Xylariaceae. Fungal Diversity 88, 1–65.
Last update: 12 August 2021